口腔颌面外科杂志2025，Vol.35Issue(6)：456-462,7.DOI:10.12439/kqhm.1005-4979.2025.06.005
白藜芦醇联合NVP-BEZ235对口腔黏膜恶性黑色素瘤抑制作用的实验研究
An experimental study on the inhibitory effect of resveratrol combined with NVP-BEZ235 on oral mucosal malignant melanoma
刘中洋 1杨志恒 1宋宁扬 1李乔乔 1宋继宏 1朴松林1
作者信息
- 1. 哈尔滨医科大学附属第一医院口腔颌面外科,哈尔滨 150001
- 折叠
摘要
Abstract
Objective:To investigate the effects of resveratrol(Res)combined with NVP-BEZ235(BEZ235)on the proliferation,invasion,and migration of the oral mucosal malignant melanoma(OMM)cell line Mm9h-1,and to explore the underlying mechanisms.Methods:Cell viability after drug treatment was detected using the CCK8 assay to determine drug concentrations and group assignments.The following groups were established for 48-hour treatment:Control group,100 µmol/L Res group,1.0 µmol/L BEZ235 group,and combination group(C1 group:100 µmol/L Res+1.0 µmol/L BEZ235).The scratch wound healing assay and Transwell assay were used to evaluate cell migration and invasion abilities,respectively.Hoechst staining was applied to observe cell death morphology,and Western blotting was performed to detect the expression levels of apoptosis-related proteins.Results:Both Res and BEZ235 alone inhibited multiple biological behaviors of Mm9h-1 cells.Compared with the Control,100 µmol/L Res,and 1.0 µmol/L BEZ235 groups,the C1 group showed significantly reduced cell viability,invasion,and migration capabilities,along with significantly upregulated expression of apoptosis-related proteins.Conclusion:The combination of Res and BEZ235 inhibits Mm9h-1 cell survival,invasion,and migration more effectively than either agent alone,and more potently induces apoptosis.关键词
白藜芦醇/NVP-BEZ235/口腔黏膜恶性黑色素瘤/细胞凋亡Key words
resveratrol/NVP-BEZ235/oral mucosal malignant melanoma/cell apoptosis分类
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刘中洋,杨志恒,宋宁扬,李乔乔,宋继宏,朴松林..白藜芦醇联合NVP-BEZ235对口腔黏膜恶性黑色素瘤抑制作用的实验研究[J].口腔颌面外科杂志,2025,35(6):456-462,7.
