林业科学研究2025，Vol.38Issue(6)：1-9,9.DOI:10.12403/j.1001-1498.20250066
核桃JrWOX4b基因在'84K'杨中的异源过表达效应分析
Regulatory Effects of Walnut JrWOX4b Gene Overexpression on Poplar Growth and Development
摘要
Abstract
[Purpose]To systematically investigate the effects of overexpressing the walnut JrWOX4b gene on adventitious root formation,stem,and leaf development in Populus'84K',and to preliminarily explore the underlying molecular mechanisms.[Methods]Previously generated JrWOX4b heterologous overex-pression transgenic'84K'poplar lines and wild-type plants were used.Phenotypic traits and tissue struc-tures of adventitious roots,stems,and leaves were analyzed.Transcriptome sequencing and bioinformatic analyses were employed to study gene-expression patterns.[Results]Phenotypic data revealed that over-expression of JrWOX4b significantly altered the root architecture of transgenic'84K'poplar,notably in-creasing total root length and total root surface area.Conversely,plant height and basal stem diameter were significantly reduced,and leaves exhibited pronounced adaxial curling.Histological observations re-vealed a marked increase in cambium cell layers in transgenic lines.In leaf main veins,the proportion of vascular bundles increased,accompanied by irregular organization of both phloem and xylem cells.RNA-seq identified 1,535 differentially expressed genes(DEGs)in stems between transgenic and wild-type plants.GO enrichment analysis revealed that DEGs were mainly associated with multicellular organ devel-opment,developmental processes,and tissue structure development.KEGG enrichment analysis indic-ated that DEGs were significantly enriched in pathways including phenylalanine,tyrosine,and tryptophan,interconversion of pentose and glucuronate,and phenethylamine metabolism.[Conclusion]Overexpres-sion of JrWOX4b significantly promotes adventitious root development in Populus of'84K',reduces plant height,and induces adaxial leaf curling.Preliminary analysis of the enriched metabolic pathways of DEGs in stems provides insights into the molecular regulatory mechanisms of JrWOX4b in root,stem,and leaf development,and offers valuable genetic resources for precision breeding through molecular approaches.关键词
核桃/不定根/生长发育/转录组/次生生长Key words
walnut/adventitious root/growth and development/transcriptome/secondary growth分类
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张普,雷夏烁,高瑞雪,常英英,宋晓波,裴东..核桃JrWOX4b基因在'84K'杨中的异源过表达效应分析[J].林业科学研究,2025,38(6):1-9,9.基金项目
核桃JrBGLU12基因介导复幼促进不定根发生的分子机制(32101479)国家级 （32101479）
