林业科学研究2025，Vol.38Issue(6)：10-20,11.DOI:10.12403/j.1001-1498.20250073
美国白蛾内向整流钾通道Kir3基因功能分析
Functional Analysis of the Inwardly Rectifying Potassium Channel Kir3 Gene in Hyphantria cunea
摘要
Abstract
[Objective]To investigate the effects of the inwardly rectifying potassium channel Kir3 gene in the Hyphantria cunea growth and development,and to offer novel molecular targets for sustainable pest control.[Method]The open reading frame(ORF)of HcKir3 gene was cloned.Quantitative reverse tran-scription PCR(qRT-PCR)was used to analyze HcKir3 expression levels in different developmental stages and tissues of H.cunea.Additionally,a prokaryotic expression system of HcKir3 synthesized in vitro was constructed to evaluate the silencing efficiency of RNA interference(RNAi)and developmental parameters in H.cunea.[Result]The ORF of HcKir3 was 1 734 bp in length,encoding a 577-amino acid protein.Gene expression analysis revealed that significantly higher HcKir3 transcript levels in eggs(4.86-fold higher than that in 2nd-instar larvae)and the hindgut(5,874.33-fold higher than in head tissue).The prokaryotic ex-pression vector of HcKir3 was successfully constructed.Additionally,dsRNA expressed by bacteria effect-ively silenced the HcKir3 and affected the growth and development of H.cunea.The larvae injected with dsHcKir3 showed significantly reduction in food consumption,body weight and fecal moisture rate.The ex-cretion rate was significantly lower than the control group in 48 h.Prepupae injected with dsHcKir3 in-creased pupal malformation rates(48.15%)with severe wing defects.Injection into pupae severely disrup-ted adult eclosion,resulting in abnormal wings and malformation rates of 70.97%(females)and 56.67%(males).[Conclusion]The HcKir3 gene is critical for the growth,development,and physiological metabol-ism of H.cunea,specifically regulating wing development.These results offer a new perspective for eco-friendly control strategies against H.cunea.关键词
内向整流钾通道/RNA干扰/美国白蛾/生长发育Key words
Inwardly Rectifying Potassium Channel/RNA interference/Hyphantria cunea/growth and development分类
农业科技引用本文复制引用
王希成,简福瑞,孙丽丽,曹传旺..美国白蛾内向整流钾通道Kir3基因功能分析[J].林业科学研究,2025,38(6):10-20,11.基金项目
国家自然科学基金项目(32171791) （32171791）
