环境与职业医学2025，Vol.42Issue(12)：1480-1490,11.DOI:10.11836/JEOM25202
基于非靶向代谢组学探究低氧重复冲刺训练前后优秀运动员的代谢变化特征
Metabolic change characteristics before and after repeated-sprint training in hypoxia based on non-targeted metabolomics in elite athletes
摘要
Abstract
[Background]Repeated-sprint training in hypoxia(RSH)can improve athletic performance and reduce body fat in obese individuals.However,studies characterizing metabolic changes experi-enced by elite athletes before and after RSH are currently scarce in China. [Objective]To characterize the plasma metabolic profile alterations in elite athletes induced by a two-week RSH intervention using untargeted metabolomics,and to analyze the associations between differential metabolites and indices of blood lipids and body composition. [Methods]Blood samples from 11 elite athletes before and after 2 weeks of RSH were collected for metabolomics analysis and blood lipid analysis.Plasma metabolites were detected by liquid chromatographymass spectrometry(LC-MS)and gas chromatography-mass spectrometry(GC-MS)combined technology,and the differential metabolites before and after training were screened out by differential analysis.Key metabolic pathways were identified by Kyoto Encyclopedia of Gene and Genome Database(KEGG)enrichment analysis.Then,the corre-lations of blood lipid indexes and body composition indexes with differential metabolites before and after training were evaluated. [Results]Finally 20 differential metabolites were identified,among which 17 lipid metabolites were significantly up-regulated,and amino acids and their derivatives(1 type)and sugar derivatives(2 types)were significantly down-regulated.The results of KEGG enrichment analysis showed that the differential metabolites were mainly involved in three pathways closely related to RSH,namely hypoxia inducible factor 1-alpha(HIF-1)signaling pathway,cyclic adenosine monophosphate(cAMP)signaling pathway,and sphingolipid signaling path-way.The post-training analysis revealed significant increases in total cholesterol(P<0.05)and high-density lipoprotein cholesterol(HDL-C)(P<0.001),as well as a significant decrease in triglycerides(P<0.01).There were minor decreases in body weight,body fat,body fat percentage,fat mass index(FMI),and fat-free mass(FFM)(P>0.05).After training,total cholesterol was negatively correlated with phos-phatidylinositol(16:0/22:4)and D-fructose,HDL-C was negatively correlated with D-fructose and β-D-glucuronic acid,while body fat,body fat percentage,and FMI showed positive correlations with multiple carnitine fatty acid derivatives. [Conclusion]The 2-week RSH promotes the decomposition of lipid metabolites,increases the utilization of fatty acids,and reduces the accumulation of carbohydrate metabolites in elite athletes,which has a positive effect on the health of blood lipid metabolism.It may also help elite athletes regulate body fat by promoting lipid metabolism.Further research is needed in the future to validate these findings.关键词
非靶向代谢组学/低氧重复冲刺训练/差异代谢物/脂质代谢/身体成分/运动员Key words
non-targeted metabolomics/repeated-sprint training in hypoxia/differential metabolite/lipid metabolism/body composition/athlete分类
医药卫生引用本文复制引用
王玺,黄琳,邱俊,高炳宏,马艳红..基于非靶向代谢组学探究低氧重复冲刺训练前后优秀运动员的代谢变化特征[J].环境与职业医学,2025,42(12):1480-1490,11.基金项目
上海市"科技创新行动计划"社会发展科技攻关项目(22dz1204600) （22dz1204600）
