环境与职业医学2025，Vol.42Issue(12)：1497-1503,7.DOI:10.11836/JEOM25225
2012-2024年中山市人工水环境嗜肺军团菌耐药性及基因特征
Antibiotic resistance and genetic characteristics of Legionella pneumophila isolated from artifi-cial water environment in Zhongshan from 2012 to 2024
摘要
Abstract
[Background]There is currently limited research on the antibiotic resistance of Legionella pneu-mophila in China.Establishing analytical methods for antibiotic resistance phenotypes and resis-tance genes of Legionella pneumophila in artificial water environment is of great significance. [Objective]To investigate the antibiotic resistance characteristics of Legionella pneumophila iso-lated from artificial water environment in Zhongshan,and to provide a scientific basis for the prevention and control of Legionella infection. [Methods]A total of 295 strains of Legionella pneumophila isolated from central air conditioning systems and hot water systems in Zhongshan from 2012 to 2024 were collected.The minimum inhibitory concentrations(MIC)of antimicrobial agents against the strains were determined using the E-test method.Whole genome sequencing combined with bioinformatics techniques was used to analyze the antibiotic resistance genes of 16 strains of Legionella pneumophila.The phy-logenetic evolutionary relationships between these strains and clonal strains from different sources worldwide were analyzed based on whole genome single nucleotide polymorphisms(wgSNPs). [Results]The antimicrobial susceptibility testing results showed that the highest resistance rate of Legionella pneumophila was to ery-thromycin(ERY)(56.9%),followed by azithromycin(AZM)(27.1%)and levofloxacin(LEV)(12.2%).The antibiotic resistance rates to ri-fampicin(RIF)and cefotaxime(CTX)were low,and the strains were fully susceptible to clarithromycin(CLA),ciprofloxacin(CIP),and doxycycline(DOX).Compared to the isolates from the hot water systems,those from the central air conditioning systems exhibited higher resistance rates to ERY(70.9%vs.28.1%)and AZM(34.2%vs.12.5%),and the differences were statistically significant(χ2=48.226,P<0.001;χ2=15.388,P<0.001).The MIC values of Lp1 isolates for four antibiotics(AZM,CLA,DOX,and CTX)were significantly higher than those of Lp2-14 isolates(Z=-3.490,P<0.001;Z=-3.035,P<0.05;Z=-2.662,P<0.05;Z=-2.973,P<0.05).The MIC values of Lp2-14 isolates for RIF were significantly higher than those of Lp1 isolates(Z=-3.190,P<0.05).The strains isolated from 2019-2024 exhibited higher MIC values for both CLA and DOX than those isolated from 2012-2018(Z=-4.847,P<0.001;Z=-6.057,P<0.001).Whole genome sequencing analysis showed that the 16 strains of Legionella pneumophila carried antibiotic resistance genes for aminoglycosides,macrolides,cephalosporins,and penicillins.The phylogenetic analysis showed that Legionella pneumophila in this region exhibited high genetic diversity and was consistent with the epidemic trends of strains from other regions worldwide. [Conclusion]The isolates of Legionella pneumophila in this study are highly susceptible to most antimicrobial agents.However,the an-tibiotic resistance should not be ignored,and continuous monitoring of antibiotic resistance is necessary.关键词
嗜肺军团菌/药敏试验/全基因组测序/耐药基因/系统发育分析Key words
Legionella pneumophila/antimicrobial susceptibility testing/whole genome sequencing/antibiotic resistance gene/phylogenetic analysis分类
医药卫生引用本文复制引用
刘绮明,袁展红,吴灿权,仇绮琳,郑悦康..2012-2024年中山市人工水环境嗜肺军团菌耐药性及基因特征[J].环境与职业医学,2025,42(12):1497-1503,7.基金项目
中山市社会公益与基础研究专项资金项目(2024B1097) （2024B1097）
