临床输血与检验2025，Vol.27Issue(6)：775-782,8.DOI:10.3969/j.issn.1671-2587.2025.06.005
氢气抑制单采血小板活化和凋亡的作用研究
Inhibitory Effects of Hydrogen on Activation and Apoptosis in Stored Apheresis Platelets
摘要
Abstract
Objective To explore the effect and mechanism of hydrogen(H2)on apheresis platelets activation and apoptosis.Methods 10 apheresis platelets were divided equally into 16 small bags and randomly divided into an H2 group and a control group.The H2 group was added with hydrogen to saturation and supplemented with hydrogen daily.All samples were stored under agitation at(22±2)℃for 8 days,the platelet count was tested,and the CD62P,PAC-1,apoptosis were detected by flow cytometry daily.The Caspase-3,Caspase-9,Bax,and Bcl-2 proteins were detected by Western blotting.Results There was no significant difference in platelet count during the preservation period(1～8 d).From the fifth day,the apoptosis rate in H2 group was significantly lower than that in the control group(20.29±4.32 vs 33.06±8.01,P＜0.001).From the sixth day,the expression of CD62P in H2 group was lower than that in the control group(24.94±5.53 vs 47.14±10.41,P＜0.001),and the expression of PAC-1 in H2 group was lower than that in the control group(14.89±5.98 vs 19.41±5.17,P＜0.05).Western blotting results showed that from the sixth day,the expression of cleaved Caspase-9 and cleaved Caspase-3 in the H2 group was lower than that in the control group(P＜0.001).From the seventh day,the expression of Bax in the H2 group was lower than that in the control group(P＜0.001),while Bcl-2 was higher than that in the control group(P＜0.05),the ratio of Bax/Bcl-2 was lower than that of the control group.Conclusion Hydrogen can reduce platelet activation and apoptosis,improve the quality of apheresis platelet storage.关键词
氢气/血小板保存损伤/活化/凋亡Key words
Hydrogen/Platelet storage lesion/Activation/Apoptosis分类
医药卫生引用本文复制引用
李津杞,秦爱华,任瑜菲,唐河山,陈翠敏,钱宝华,臧艳..氢气抑制单采血小板活化和凋亡的作用研究[J].临床输血与检验,2025,27(6):775-782,8.基金项目
本课题受海军军医大学第一附属医院"234学科攀峰计划"(No.2020YXK036)资助 （No.2020YXK036）
