临床输血与检验2025，Vol.27Issue(6)：809-813,5.DOI:10.3969/j.issn.1671-2587.2025.06.010
用于酸放散试验的压积红细胞容量对新生儿溶血病检测结果的影响研究
Study on the Effect of Packed Red Blood Cells Volume on the Result of Acid Elution Test for Neonatal Hemolytic Disease
摘要
Abstract
Objective To analyze the effect of different packed red blood cells(pRBCs)volume on the result of acid elution test,for optimizing the sample volume in neonatal hemolytic disease detection.Methods One hundred and fifty specimens with suspected neonatal hemolytic disease were collected from February 2022 to December 2022 for hemolytic triple test.We identified the elution agglutination intensity of specimens confirmed ABO neonatal hemolytic disease under four different pRBCs volume(A:250 μL,B:500 μL,C:750 μL,D:1 000 μL).Cochran's Q test was used to analyze the differences in overall positive rates among four groups.McNemar's test was applied for multiple comparisons.The Friedman test was used to analyze the differences in the overall distribution of agglutination intensity,and the Wilcoxon test was applied for multiple comparisons.Results In 118 cases of confirmed ABO neonatal hemolytic disease,the positivity rate was 94.92％in group A,and 100％in groups B,C,and D(P＜0.05).There was no significant difference in positive rate between group A and groups B/C/D(corrected P＞0.008 3).The overall difference(P＜0.001)and the multiple comparison difference(P＜0.008 3 after correction)in the rank distribution of agglutination intensity for the four groups were statistically significant,and the agglutination intensity increased with the increase of pRBCs volume.Conclusion We can reduce the pRBCs volume from 1 000 μL to 500 μL in acid elution test,ensuring the accuracy of the test results and saving blood sample volume.关键词
ABO新生儿溶血病/酸放散试验/压积红细胞/凝集强度Key words
ABO Neonatal hemolytic disease/Acid elution test/Packed red blood cells/Agglutination intensity分类
医药卫生引用本文复制引用
沈经纬,张文梅,胡家梅,张娜,杨毅梅,马顺高..用于酸放散试验的压积红细胞容量对新生儿溶血病检测结果的影响研究[J].临床输血与检验,2025,27(6):809-813,5.基金项目
本课题受云南省教育厅科学研究基金项目(No.2019J0806)资助 （No.2019J0806）
