棉花学报2025，Vol.37Issue(5)：415-428,14.DOI:10.11963/cs20250048
旱地棉PHD的全基因组挖掘及在非生物胁迫下的表达分析
Genome-wide identification of PHD in Gossypium aridum and its expression analysis under abiotic stresses
摘要
Abstract
[Objective]Gossypium aridum exhibits excellent stress tolerance.Plant homeodomain(PHD)proteins play a crucial role in regulating plant responses to abiotic stress.This study aimed to mine PHD genes in the whole genome of G.aridum,and analyze their expression patterns under abiotic stress.[Methods]G.aridum genome was mined for PHD using bioinformatics methods.The physicochemical properties,conserved domains and motifs,phylogenetic relationships of PHD proteins,cis-acting elements and transcription factor binding sites in the promoter region were predicted and analyzed.The expression levels of GarPHD genes under salt,drought,and low-temperature stresses were analyzed using quantitative real-time polymerase chain reaction(qRT-PCR).[Results]A total of 38 GarPHD genes were identified in G.aridum.The PHD domain of GarPHD proteins contains the consistent sequence:X(0-1)-C-X(1-2)-C-X(8-13)-C-[DESANQ]-X(1-2)-C-X(4)-H-X(2)-C-X(9-16)-[WYF]-X-C-X(2)-C.PHD proteins of G.aridum,G.tomentosum,and G.barbadense were divided into 5 subgroups.The promoter regions of GarPHD contain cis-acting elements related to growth and development,plant hormones,and stress responses,as well as binding sites for 33 transcription factors.The qRT-PCR analysis revealed significant changes in the expression levels of certain GarPHD genes in response to salt,drought,and low-temperature stress.Specifically,GarPHD 19 was found to respond to both low-temperature and drought stress,GarPHD31 responded to both salt and drought stress.[Conclusion]Thirty-eight PHD genes were identified in G.aridum.Some GarPHD responded to salt,drought,and low-temperature stress,which lays the foundation for further research on the function of GarPHD genes.关键词
旱地棉/非生物胁迫/PHD/基因表达量/全基因组鉴定Key words
Gossypium aridum/abiotic stress/PHD/gene expression/genome-wide identification引用本文复制引用
吕宇,韩文兵..旱地棉PHD的全基因组挖掘及在非生物胁迫下的表达分析[J].棉花学报,2025,37(5):415-428,14.基金项目
国家重点研发计划(2024YFD1200300) （2024YFD1200300）
安徽省财政农业科技成果转化项目(2025AHSZH28) （2025AHSZH28）
国家现代农业产业技术体系建设专项"国家棉花产业技术体系安徽综合试验站" （）
安徽省现代农业产业技术体系建设专项"安徽省油料作物产业技术体系"(ahxdny-4) （ahxdny-4）
