现代农业研究2025，Vol.31Issue(9)：38-45,8.
一株耐盐碱产IAA菌株的筛选、鉴定及培养条件优化
Screening,Identification,and Culture Condition Optimization of a Salt-Alkali Tolerant IAA-Producing Bacterial Strain
摘要
Abstract
Soil salinization makes it difficult for plants to synthesize sufficient indole-3-acetic acid(IAA)to meet their needs.In the context of widespread use of pesticides and chemical agents,we opted for a more environmentally friendly approach using microbial inoculants.In this study,a bacterial strain capable of tolerating salt-alkali stress and producing IAA was isolated and identified as Enterobactersp.through screening for salt-alkali tolerance,siderophore production,nitrogen fixation,and IAA production.The bacterial strain was cultured and applied to rice seedlings under salt-alkali stress.After 21 days,physiological indicators of the rice seedlings were measured.The results showed that the bacterial treatment significantly enhanced root length,plant height,and the activities of various antioxidant enzymes in rice under salt-alkali stress.Through single-factor optimization of fermentation conditions,the optimal parameters were determined as follows:15 g/L starch,10 g/L peptone,4 g/L calcium chloride,0.3 g/L tryptophan,3%inoculation volume,3 days of cultivation,and initial pH of 6.Under these conditions,IAA production increased by 98.45%.The salt-alkali tolerant IAA-producing Enterobactersp.,after fermentation optimization,demonstrated significantly enhanced IAA production.Its application effectively promoted the growth and physiological performance of rice seedlings under salt-alkali stress,providing a green and sustainable microbial solution to address IAA deficiency in plants caused by soil salinization,with both theoretical value and agricultural application prospects.关键词
水稻/根际促生菌/盐碱胁迫/促生效果Key words
rice/plant growth-promoting rhizobacteria/salt-alkali stress/growth-promoting effect分类
资源环境引用本文复制引用
杨嘉仪,李昂,宋俊杰,梅艳湘,高泽泓,马书荣..一株耐盐碱产IAA菌株的筛选、鉴定及培养条件优化[J].现代农业研究,2025,31(9):38-45,8.基金项目
国家自然科学区域创新发展联合基金项目"苏打盐碱地根际微生物提高饲料作物苜蓿、玉米盐碱抗性的作用机制"(项目编号:U23A20151) （项目编号:U23A20151）
黑龙江省大创项目(项目编号:S202510225400). （项目编号:S202510225400）
