农业大数据学报2025，Vol.7Issue(4)：532-542,11.DOI:10.19788/j.issn.2096-6369.100063
禽腺病毒多重TaqMan荧光定量PCR检测数据集
Detection Dataset of Multiple Taq-Man Real-time PCR for Fowl Aviadenoviruses
摘要
Abstract
Several serotypes of fowl aviadenoviruses were the aetiologic agent of hydro-pericardium-hepatitis syndrome(HHS),inclusion body hepatitis(IBH)and gizzard erosion(GE)in chicken farms.Outbreaks of these had been documented in multiple provinces of China in 2015,causing significant economic losses.Moreover,this disease had diverse transmission modes with no obvious seasonality,which was a mortality rate reported to be 30%-80%.All these had imposed more requirements on the biosecurity prevention and control level of various chicken farms.How to protect the health of the chicken flock and ensure the normal production of chicken farms was a key focus for chicken farms and related scientific research institutions.This dataset included clinical sample collection data,gene sequence comparison data of 12 serotypes of fowl aviadenoviruses,fluorescent probe primer sequence data,reaction system optimization data for the Multiple Taq-Man real-time PCR detection of fowl aviadenoviruses,and a data of 161 clinical samples tested simultaneously with industry standards.Data showed that this method could specifically detect FAdV-4,FAdV-8a and FAdV-8b,with no cross-reactivity with other serotypes of fowl aviadenoviruses,as well as NDV,AIV H9 subtype,IBV,etc.It had a minimum detection limit of 17 copies/μL and good stability,and the comparison result between the established method and the trade standard was 100.00%.In addition,the infection rate of fowl aviadenoviruses in chicken farms in Tianjin and surrounding areas was relatively low.This dataset could be used for the detection of FAdV-4,FAdV-8a and FAdV-8b,providing data support for epidemiological investigation and early rapid screening of this disease in chicken farms.It could also be widely applied in the theoretical research and practical application of biosafety prevention and control technologies for other epidemic diseases in chicken farms..关键词
禽腺病毒/多重荧光定量PCR/快速筛查/数据集Key words
fowl aviadenoviruses/the Multiple Taq-Man real-time PCR/rapid screening/Detection dataset引用本文复制引用
王利丽,任卫科,田春利,鄢明华,师延峰,赵海明,李富强,崔松奇,郭笑然,李娥,孙慧中,李鹏飞..禽腺病毒多重TaqMan荧光定量PCR检测数据集[J].农业大数据学报,2025,7(4):532-542,11.基金项目
中央级公益性科研院所基本科研业务费院级统筹项目:动物疫病数据中心 （）
天津市科技计划项目(23YDTPJC00050) （23YDTPJC00050）
畜禽种质资源创新及高效繁育技术研究(2025ZYCX004). （2025ZYCX004）
