山东农业大学学报（自然科学版）2025，Vol.56Issue(6)：997-1009,13.DOI:10.3969/j.issn.1000-2324.2025.06.010
异源表达甘菊ClDEL1基因影响拟南芥花瓣形态建成
Heterologous Expression of Chrysnathemum lavandulifolium ClDEL1 Gene Affects Petal Morphogenesis in Arabidopsis thaliana
摘要
Abstract
Chrysanthemum is a typical species of the genus Chrysanthemum with capitula as its ornamental parts.Its inflorescence development is controlled by a complex gene regulatory network,in which cell cycle regulators play an important role in the formation of inflorescence diversity.DEL1(DP-E2F-like 1),a member of the E2F transcription factor family,has been demonstrated to play a significant role in cell cycle regulation and plant growth and development in Arabidopsis thaliana.However,there have been no reports to date on the biological function of the homologous gene DEL1 in Chrysanthemums.Using Chrysanthemum lavandulifolium as the experimental material,a model plant for molecular research in the Chrysanthemum genus,this study clones and identifies the homologous gene ClDEL1 in Chrysanthemum lavandulifolium,with reference to the DEL1 protein sequence of Arabidopsis.ClDEL1 contains two conserved domains,localized in the nucleus.Through RT-qPCR,this study analyzes the expression pattern of ClDEL1 in the roots,stems,leaves,ray florets,and tubular florets of Chrysanthemum lavandulifolium.The results show that ClDEL1 exhibits the highest expression level in the ray florets,followed by stems,leaves,and disc florets,while the lowest in roots.Heterologous overexpression of ClDEL1 has no significant effect on the rosette leaf morphology of Arabidopsis thaliana,but it significantly inhibits the growth of Arabidopsis thaliana petals,resulting in a reduction in petal area.Based on the phenotypic analysis of Arabidopsis thaliana AtDEL1 mutants and their complementation lines,this study confirms that ClDEL1 in Chrysanthemum lavandulifolium and AtDEL1 are functionally conserved homologous genes,both exhibiting similar biological functions in regulating cell division and expansion.Cytological evidence further indicates that ClDEL1 is involved in regulating petal morphogenesis in Arabidopsis thaliana by inhibiting cell division and promoting cell expansion.关键词
甘菊/花瓣发育/ClDEL1/细胞分裂/细胞扩张Key words
Chrysanthemum lavandulifolium/petal development/ClDEL1/cell division/cell expansion分类
农业科技引用本文复制引用
张梅,刘波,王亚慧,张鹏,王志敏,包志龙,马方放..异源表达甘菊ClDEL1基因影响拟南芥花瓣形态建成[J].山东农业大学学报（自然科学版）,2025,56(6):997-1009,13.基金项目
泰山学者青年专家项目(tsqn201909073) （tsqn201909073）
