山西农业大学学报（自然科学版）2025，Vol.45Issue(6)：12-22,11.DOI:10.13842/j.cnki.issn1671-8151.202508012
辣椒CaFLK基因克隆及表达分析
Cloning and expression analysis of CaFLK gene in pepper
摘要
Abstract
[Objective]This paper aimed to investigate the potential biological functions of the CaFLK gene in pepper.[Meth-ods]Using'Jinjiao 204'as experimental material,the CaFLK gene and its promoter sequence was successfully cloned via PCR technology.Systematic bioinformatics analysis was performed on the obtained sequences.Furthermore,the differences in the transcriptional level of this gene across various tissues and its dynamic expression changes under different stresses were ana-lyzed.[Results]The full length of the cloned CaFLK gene was 1452 bp,encoding 483 amino acid residues and containing three KH conserved domains.Analysis of physicochemical properties indicated that the CaFLK protein had a molecular weight of 51.75 kDa and an isoelectric point of 4.83,classifying it as an acidic protein.Its instability index was 59.18,categorizing it as an unstable protein,and its grand average of hydrophilicity index was 4.83,indicating it is a hydrophilic protein.The protein was predicted to contain 38 phosphorylation sites and 2 N-glycosylation sites.It lacked a signal peptide and transmembrane do-mains.Random coils and α-helices were the key structural components,dominating the secondary and tertiary structures of this gene.Phylogenetic analysis showed that CaFLK shared up to 95%homology with Solanaceae crops such as tomato(Solanum lycopersicum),sticky nightshade(Solanum verrucosum),goji berry(Lycium barbarum),and tobacco(Nicotiana tabacum).A 2000 bp CaFLK promoter sequence was cloned,and cis-acting element prediction identified various stress and hormone re-sponse elements within this region.Protein interaction prediction suggested that five flowering-related proteins interact with CaFLK.Quantitative real-time PCR(qRT-PCR)results showed that the CaFLK gene has a high expression level in leaves and exhibited elevated expression level under low temperature,ABA and MeJA treatment.[Conclusion]The CaFLK gene was regulated by different stresses and hormones and may interact with flowering-related proteins.These findings provided insights for its functional characterization.关键词
辣椒/FLK基因/生物信息学/表达分析Key words
Pepper/FLK gene/Bioinformatics/Expression analysis分类
农业科技引用本文复制引用
王梦,柳璐,郑晓美,臧巧路..辣椒CaFLK基因克隆及表达分析[J].山西农业大学学报（自然科学版）,2025,45(6):12-22,11.基金项目
山西省重点研发计划项目(202402140601003-02) （202402140601003-02）
山西农业大学博士科研启动项目(2022BQ13) （2022BQ13）
蔬菜花卉种质资源创新与利用山西省重点实验室2024年度开放课题(SCHHZDSYS2024-14) （SCHHZDSYS2024-14）
山西省基础研究计划(自由探索类)青年科学研究项目(202203021212454) （自由探索类）
山西省博士毕业生来晋工作奖励科研项目(SXBYKY2022060) （SXBYKY2022060）
山西省科技创新人才团队(202304051001018) （202304051001018）
