山西农业大学学报（自然科学版）2025，Vol.45Issue(6)：23-30,8.DOI:10.13842/j.cnki.issn1671-8151.202411024
矮化基因dw1、dw2和dw3对高粱株高的贡献效应分析
Analysis of the contribution effects of dwarf genes dw1,dw2 and dw3 on sorghum plant heights
摘要
Abstract
[Objective]This study aimed to investigate the contribution of single gene or combined dwarfing genes dw1,dw2 and dw3 to reducing sorghum plant height.[Methods]Molecular markers were developed based on known variation sites in the Dw1,Dw2 and Dw3 alleles.Plant heights and Dw alleles were analyzed in 14 sorghum accessions and F2 population derived from a cross between 8R252(Dw1Dw2Dw3)and 8R387(dw1dw2dw3)to assess the influence of the three dwarfing genes and their allelic combinations on plant heights.[Results]Practical molecular markers were developed based on mutations,insertions,and deletions in the coding regions of the Dw1,Dw2 and Dw3 genes.In the natural sorghum population,plant-height varied sig-nificantly even when the Dw1,Dw2 and Dw3 alleles were identical.However,the plant heights gradually decreased with the ac-cumulation of more dwarfing alleles.Analysis of the F2 population,which contained eight different allelic combinations,re-vealed the following patterns:when one gene was mutated,the reduction in plant height followed the order Dw1Dw2dw3>dw1Dw2Dw3>Dw1dw2Dw3;When two gene were mutated,the reduction followed dw1Dw2dw3>Dw1dw2dw3>dw1dw2Dw3;and when all three genes were mutated,plant height was the shortest.[Conclusion]The three cloned dwarfing genes in sorghum contribute differently to plant height reduction,with the magnitude of reduction following the order of dw3>dw1>dw2.关键词
高粱/株高/矮化基因/分子标记Key words
Sorghum/Plant height/Dwarfing gene/Molecular marker分类
农业科技引用本文复制引用
王春语,王平,丛玲,李金红,白春明,张丽霞..矮化基因dw1、dw2和dw3对高粱株高的贡献效应分析[J].山西农业大学学报（自然科学版）,2025,45(6):23-30,8.基金项目
辽宁省"种质创新藏粮于技"重大专项(2023JH1/10200009) （2023JH1/10200009）
国家现代农业产业技术体系项目(CARS-06-14.5-A3) （CARS-06-14.5-A3）
辽宁省农业科学院院长基金项目(2025XKJS8504) （2025XKJS8504）
