生态学杂志2025，Vol.44Issue(12)：3891-3897,7.DOI:10.13292/j.1000-4890.202512.034
互花米草入侵对福建省滨海湿地土壤固氮菌丰度的影响
Effect of Spartina alterniflora invasion on the abundance of diazotrophs in coastal wetland soils of Fujian Province
摘要
Abstract
Diazotrophs are the primary source of bioavailable nitrogen in natural ecosystems,making the elucidation of their abundance paramount for effective soil nitrogen management.To reveal the response of soil diazotroph abun-dance to Spartina alterniflora invasion and its key controlling factors,we collected soil samples from eight typical coastal wetlands in Fujian Province and employed real-time quantitative PCR to measure the abundance of nifH genes in bare tidal flats and S.alterniflora-invaded wetland soils.The results showed that S.alterniflora invasion in-creased the contents of soil organic carbon(SOC),ammonium(NH4+-N),nitrate(NO3--N),sulfate(SO42-),microbial biomass carbon(MBC),microbial biomass nitrogen(MBN),salinity,and Cl-,while decreased soil pH.The copy number of nifH gene in S.alterniflora-invaded wetland soils was significantly higher than that in bare tidal flat soil,suggesting that S.alterniflora invasion significantly increased soil diazotroph abundance.The abun-dance of nifH gene was significantly negatively correlated with soil pH and positively correlated with SOC,NH4+-N,NO3--N,and MBN.Path analysis indicated that S.alterniflora invasion affected the abundance of nifH genes both directly and indirectly through SOC and MBN.Stepwise regression analysis revealed that MBN and SO42-explained most of the variations in nifH gene abundance,with MBN being the most crucial environmental factor controlling nifH gene abundance.In summary,S.alterniflora invasion significantly increases the abundance of soil nifH genes in typical coastal wetlands of Fujian Province,with MBN being the most critical environmental factor regulating nifH gene abundance.关键词
滨海湿地/植物入侵/固氮菌/nifHKey words
coastal wetland/plant invasion/diazotroph/nifH引用本文复制引用
叶悦龄,左婷,陈琦润,杨平,叶桂萍,林永新..互花米草入侵对福建省滨海湿地土壤固氮菌丰度的影响[J].生态学杂志,2025,44(12):3891-3897,7.基金项目
国家自然科学基金项目(42407416)、福建省自然资源科技创新项目(KY-090000-04-2022-012)和闽江学院引进人才预研项目(MJY20012)资助. （42407416）
