生物技术进展2025，Vol.15Issue(6)：969-976,8.DOI:10.19586/j.2095-2341.2025.0063
Fab合成噬菌体文库在抗体发现领域的应用进展
The Appliation Advance of Fab Synthetic Libraries with Phage Display in Antibody Discovery
闵雪 1陶维红2
作者信息
- 1. 河南大学抗体药物开发技术国家地方联合工程实验室,河南 开封 475004
- 2. 河南大学抗体药物开发技术国家地方联合工程实验室,河南 开封 475004||上海溪长生物技术有限公司,上海 201712
- 折叠
摘要
Abstract
Antibodies have attracted significant attention in disease treatment,especially in cancer therapy,owing to their high target selectivity.However,traditional antibody discovery methods,such as hybridoma technology,are complex to operate and dependent on animal models,rendering them increasingly inadequate to meet the needs of modern large-scale antibody screen-ing.Consequently,there is an urgent need to develop novel high-throughput antibody screening approaches.Fab synthetic phage libraries offer advantages including lower cost,higher efficiency,and the capability to generate custom-specific antibodies,which is conducive to the identification of new disease targets and large-scale antibody screening.This review systematically sum-marized the research progress and clinical applications of Fab synthetic phage display libraries in terms of library design,display systems,and screening methods,with the aim of providing references for optimizing antibody screening and engineering.关键词
Fab抗体/噬菌体文库/合成文库/抗体发现Key words
Fab antibody/phage display library/synthetic library/antibody discovery分类
生物科学引用本文复制引用
闵雪,陶维红..Fab合成噬菌体文库在抗体发现领域的应用进展[J].生物技术进展,2025,15(6):969-976,8.
