生物技术进展2025，Vol.15Issue(6)：1003-1019,17.DOI:10.19586/j.2095-2341.2025.0145
赤松梢斑螟气味降解酶基因筛选及其在不同组织的表达差异研究
Study on Screening of Odorant Degradation Enzyme Genes in Dioryctria sylvestrella and its Expression Level in Different Tissues
摘要
Abstract
This study aimed to identify,classify,and analyze the tissue expression patterns of odorant-degrading enzyme(ODE)-related genes in Dioryctria sylvestrella(Lepidoptera:Pyralidae),thereby providing a theoretical basis for developing green and efficient control technologies against this pest.Four families of ODE-related genes were screened from the transcriptome data of female and male antennae of D.sylvestrella,including carboxylesterases(CXEs),aldehyde oxidases(AOXs),glutathione S-transferases(GSTs),and cytochrome P450 monooxygenases(CYPs).These genes were subjected to bioinformatics analysis,homologous sequence alignment,phylogenetic tree construction,and standardized naming.Candidate genes with relatively high FPKM values in antennae were selected for subsequent validation,and reverse transcription-quantitative polymerase chain reac-tion(RT-qPCR)was employed to analyze the tissue-specific expression profiles of the selected genes in different adult tissues.A total of 137 candidate ODE-related genes were identified,including 39 DsylCXEs,6 DsylAOXs,32 DsylGSTs,and 60 DsylCYPs.RT-qPCR results showed that 18 DsylODEs exhibited significantly higher expression levels in antennae than in other tissues.Among these antennae-enriched ODE genes,glutathione S-transferases(GSTs)and cytochrome P450 monooxygenases(CYPs)were the dominant families.This study is the first to screen and identify potential D.sylvestrella ODE genes with strong odor-de-grading capabilities,which established a foundational framework for further investigating the odor degradation mechanisms in-volved in the olfactory recognition process of D.sylvestrella through sequence characterization and gene expression profiling.关键词
赤松梢斑螟/气味降解酶/转录组/RT-qPCRKey words
Dioryctria sylvestrella/odorant degradation enzymes/transcriptome/RT-qPCR分类
生物科学引用本文复制引用
张凯鹏,王莉,唐莉,李婧,陈琪..赤松梢斑螟气味降解酶基因筛选及其在不同组织的表达差异研究[J].生物技术进展,2025,15(6):1003-1019,17.基金项目
吉林省科技发展计划项目(20230202096NC). （20230202096NC）
