摘要
Abstract
Objective To construct a composite nano-film for oral use based on biocompatible and bioactive materials with a-bundant active sites grafted with adhesion groups and functional factors,and to provide a new approach for the treatment of oral mucosal wounds through studying its mechanical and biological properties.Methods A composite nanofilm was prepared using methacrylated gelatin(GelMA),dopamine(DA),antimicrobial peptides(AMP),and calcium ions(Ca2+)as the base materials by solution spin coating.The mechanical properties were evaluated by tensile stress and swelling stress tests.The biocompatibility was detected by cell viability and CCK-8 proliferation assays.The in vitro antibacterial ability of the composite films was determined by plate inhibition tests and SEM.The in vitro hemostatic performance of the composite nanofilms was analyzed by the platelet method.The wet adhesion abili-ty was investigated by in vitro adhesion tests.Results GelMA,DA,AMP and Ca2+were selected as the base materials,and an inde-pendent nanofilm was fabricated by solution spin-coating method.The pressure tolerance reached(6.75±0.25)kPa,which was higher than human blood pressure.The maximum tensile stress was 4 MPa,close to that of human soft tissues.Cell experiments confirmed good biocompatibility.The bactericidal efficiency exceeded 99%within 2 h.It exhibited excellent wet adhesion performance in simula-ted oral moist environment.Conclusions A composite nanofilm with wet adhesion,antibacterial and hemostatic properties for oral use has been successfully constructed for oral use,providing an innovative solution for the closure and repair of oral mucosal wounds.关键词
口腔用复合纳米膜/口腔黏膜创面/甲基丙烯酰化明胶/多巴胺/湿黏接Key words
oral nanofilm/oral mucosal wound/methacrylated gelatin/dopamine/wet adhesion分类
医药卫生
