武警医学2025，Vol.36Issue(12)：1050-1054,5.
微泡造影剂在猪肝创伤性出血超声微波消融止血中的效果
Effect of microbubble contrast agent in ultrasound-guided microwave ablation for hemo-stasis in porcine traumatic liver hemorrhage
摘要
Abstract
Objective To evaluate the enhancement effect of microbubble contrast agent on the accuracy of ultrasound-guided microwave ablation for hemostasis and the hemostatic efficacy in a porcine traumatic liver hemorrhage model.Methods A randomized controlled animal trial was conducted utilizing 24 miniature pigs(weight:30-50 kg).Following establishment of a standardized liver injury model,subjects were randomly allocated to either a microbubble contrast agent group(n=12,receiving intravenous SonoVue at 0.1 ml/kg)or a saline control group(n=12,administered an equivalent volume of saline).Both groups were treated with KY2000 microwave therapy instrument(power 60 W)for ablation hemostasis under ultrasound guidance.Hemodynamic parameters-including heart rate,mean arterial pressure,and rectal temperature-were monitored continuously throughout the procedure.Postoperative liver dissection and histopathological examinations were performed.Results The experimental results showed that in the ultrasonographic examination,the group using microbubble contrast agents exhibited significantly enhanced echo intensity in the hemostatic area,with markedly improved clarity of the boundaries adjacent to normal liver tissue,facilitating precise intraoperative localization of the bleeding site.The microwave needle under ultrasound guidance rapidly reached the center of the bleeding site,with an average hemostasis time of approximately 3±0.32 minutes across different liver regions.In areas adjacent to the hepatic artery,the coagulation time in the mi-crobubble contrast agent group was significantly shorter than that in the control group[(5.50±0.52)min vs.(9.67±0.85)min,P<0.001].However,in regions near the diaphragm and gallbladder,there was no statistically significant difference in coagulation time between the two groups.Intraoperative physiological parameter monitoring revealed no statistically significant differences in heart rate,mean arterial pressure(MAP),or rectal temperature between the two groups.Nevertheless,analysis of blood pressure trends indi-cated that the microbubble contrast agent group had smaller fluctua-tions in systolic and diastolic blood pressure,suggesting a more stable hemodynamic state.Laboratory tests 24 hours postoperatively showed that the decrease in hemoglobin,reduction in platelet count,and total blood loss in the microbubble contrast agent group were significantly lower than those in the control group(P<0.05).Pathological examination revealed that both groups formed spindle-shaped coagulative necrosis zones[diameter(1.52±0.15)cm]after microwave ablation.Under optical microscopy,the degree of he-patic sinus congestion and dilation was more pronounced in the microbubble contrast agent group.Conclusions In a porcine model of traumatic liver hemorrhage,microbubble contrast agent can significantly enhance ultrasonographic visualization of the bleeding site,im-prove localization accuracy,and effectively abbreviate microwave ablation hemostasis time in critical areas such as the adjacent hepatic artery,thereby enhancing overall hemostatic efficiency.关键词
微泡造影剂/战创伤/肝出血/超声引导/微波消融Key words
microbubble contrast agent/combat trauma/liver hemorrhage/ultrasound guidance/microwave ablation分类
医药卫生引用本文复制引用
彭碧波,李胜男,高相楠,杨贵荣,宋慧娜,高永艳..微泡造影剂在猪肝创伤性出血超声微波消融止血中的效果[J].武警医学,2025,36(12):1050-1054,5.基金项目
北京市自然科学基金面上项目(7222230) （7222230）
