武汉工程大学学报2025，Vol.47Issue(6)：641-646,6.DOI:10.19843/j.cnki.CN42-1779/TQ.202508003
庆大霉素生产菌种育种研究进展
Advances in breeding technologies for gentamicin-producing strains
摘要
Abstract
This review systematically outlines the development and current research status of breeding technologies for gentamicin-producing strains,providing a detailed comparison between traditional mutagenesis breeding and modern genetic engineering approaches.In traditional breeding methods,physical mutagenesis(including ultraviolet irradiation,ion beam implantation,and ARTP)and chemical mutagenesis enhance strain yield by inducing DNA mutations.Specifically,UV mutagenesis increases shake-flask titer by 34.3%,while ARTP mutagenesis improves the titer from 1 547 U/mL to 2 280 U/mL.In modern genetic engineering,gene knockout technology successfully increases the proportion of the gentamicin C1a component from 6.5%to 94.0%by blocking specific metabolic pathways;CRISPR/Cas9 gene editing enables precise regulation of methyltransferases and glycosyltransferases;and DNA shuffling technology provides a pathway for developing novel antibiotic derivatives through homologous gene recombination.The review demonstrates that gentamicin strain breeding has transitioned from random mutagenesis to rational design.Future developments will focus on the deep integration of synthetic biology,metabolic engineering,and AI predictive models to achieve efficient bio-manufacturing of secondary metabolites like gentamicin through global metabolic network analysis.关键词
庆大霉素/诱变选育/基因工程Key words
gentamicin/mutagenesis breeding/genetic engineering分类
生物科学引用本文复制引用
丁强,龚波,向继武,卜峰,谭小芳,谭蓉,杨倩,刘佳鑫,程波,周平..庆大霉素生产菌种育种研究进展[J].武汉工程大学学报,2025,47(6):641-646,6.基金项目
湖北省重点研发计划项目(2023BBB120) （2023BBB120）
