新疆师范大学学报（自然科学版）2026，Vol.45Issue(1)：74-82,9.
沙生蜡菊qRT-PCR内参基因筛选与验证
Screening and Verification of Reference Genes for qRT-PCR in Helichrysum Arenarium(L.)Moench
摘要
Abstract
Five reference genes(ACT1,ACT7,CYP,HIS,TUB)were selected as candidate reference genes from the transcriptome data of Helichrysum arenarium,and qRT-PCR was used to detect the expression levels of candidate reference genes in different tissues and under freezing stress of Helichrysum arenarium.geNorm,NormFinder,BestKeeper and RefFinder were used to evaluate their stability.The stability of the reference gene was verified by using HaWRKY gene as the target gene.The expression of HaWRKY gene in Helichrysum arenarium was analyzed to verify the effect of reference genes on the experimental results.It was found that CYP and HIS were stably expressed in Helichrysum arenarium,but HIS was stably expressed after freezing stress.In summary,HIS can be used as an internal reference gene of Helichrysum arenarium.In this study,the suitable reference genes of Helichrysum arenarium were obtained,which provided reliable technical support for gene expression analysis of Helichrysum arenarium,and also laid a foundation for further study on the molecular mechanism of freezing tolerance and regulation of secondary metabolism.关键词
沙生蜡菊/内参基因/qRT-PCR/基因表达分析Key words
Helichrysum arenarium/Internal reference gene/qRT-PCR/Gene expression analysis分类
农业科技引用本文复制引用
刘欣欣,赖成霞,谷玉风,葛风伟..沙生蜡菊qRT-PCR内参基因筛选与验证[J].新疆师范大学学报（自然科学版）,2026,45(1):74-82,9.基金项目
新疆维吾尔自治区重点研发项目(2023B02023-1). （2023B02023-1）
