摘要
Abstract
Objective To preliminarily explore the mechanism of ethyl carbonate-induced anesthesia by detecting ATPase activity in different brain regions during various stages of anesthesia.Methods Kunming mice were randomly divided into five groups based on body weight(8 rats per group):control group,induction group,anesthesia group,recovery phase Ⅰ group and recovery phase Ⅱ group.The control group received an intraperitoneal injection of saline,while the other four groups received an intraperitoneal injection of ethyl carbonate(1000 mg/kg).Brain tissues were collected once the mice reached the corresponding state.Na+-K+-ATPase,Mg2+-ATPase,and Ca2+-ATPase activities were measured in the cortex,hippocampus,brainstem,and thalamus.Results Compared to the control group,Na+-K+-ATPase,Mg2+-ATPase and Ca2+-ATPase activities in all brain regions were decreased to varying degrees during the induction phase,with the most significant reduction observed during the anesthesia phase(P＜0.05).Na+-K+-ATPase,Mg2+-ATPase and Ca2+-ATPase activities began to recover in both recovery phase Ⅰ and recovery phase Ⅱ.By recovery phase Ⅱ,ATPase activities in all brain regions returned to normal levels(P＞0.05),except for Mg2+-ATPase and Ca2+-ATPase activities in the cortex.Conclusion The anesthetic effect of ethyl carbonate may be associated with the suppression of ATPase activity.关键词
氨基甲酸乙酯/麻醉/ATP酶Key words
Ethyl carbonate/Anesthesia/ATPase分类
医药卫生
