新疆农业大学学报2025，Vol.48Issue(2)：122-132,11.DOI:10.20088/j.cnki.jxau.2025.02.005
大丽轮枝菌锌指蛋白基因家族的系统鉴定与致病表达模式分析
Systematic Identification of the Zinc Finger Protein Gene Family and Expression Analysis during Pathogenesis in Verticillium dahliae
摘要
Abstract
The objective of this study is to systematically identify the zincfinger protein(ZFP)gene family in Verticillium dahliae,characterize its evolutionary features,and investigate the expression profile of VdZFP dur-ing host infection.Based on the whole genome sequence of V.dahliae,ZFP genes were identified using bioinfor-matics tools.Comprehensive analyses were performed,including phylogenetic tree construction,gene structure annotation,chromosomal distribution,and subcellular localization prediction.The expression pattern of VdZFP during cotton infection further validated via qRT-PCR.A total of 77 ZFPs genes were identified in V.dahliae.Phylogenetic analysis classified them into five distinct subfamilies(Ⅰ～Ⅴ),each showing significant structural and functional divergence.Chromosomal mapping revealed a non-random clustered distribution of these genes.Subcellular localization predictions indicated that 82%of ZFPs genes were nuclear-localized.qRT-PCR results demonstrated that the members of the VdZFP family may play an important role in the infection process.Among them,VdZFP16 exhibited a unique bimodal expression pattern-early suppression,followed by late activa-tion during infection,with expression down regulation to 33%of the control at 4-8 hpi and sharply up-regulated to10.57-fold at 48 hpi.These findings suggest that VdZFP16 may facilitate fungal pathogenesis through its bi-modal expression dynamics and could serve as a potential target for controlling Verticillium wilt.This study pro-vides important insights into the functional roles of ZFP genes in V.dahliae and their mechanisms during patho-gen-host interaction.关键词
大丽轮枝菌/锌指蛋白/基因家族/系统鉴定/表达分析Key words
Verticillium dahliae/zinc finger protein/gene family/phylogenetic analysis/expression analysis分类
农业科技引用本文复制引用
毕研清,吕晓迪,朱鑫宇,张国帅,李月..大丽轮枝菌锌指蛋白基因家族的系统鉴定与致病表达模式分析[J].新疆农业大学学报,2025,48(2):122-132,11.基金项目
新疆维吾尔自治区自然科学基金资助项目(2023D01E03) （2023D01E03）
新疆维吾尔自治区天山英才培养计划"三农"骨干人才(2023SNGGNT063) （2023SNGGNT063）
自治区高校基本科研业务费(XJEDU2025Z002) （XJEDU2025Z002）
