园艺学报2025，Vol.52Issue(12)：3143-3156,14.DOI:10.16420/j.issn.0513-353x.2024-0997
苹果肌醇-1-磷酸合成酶基因MdMIPS1参与高温胁迫应答的功能分析
The Inositol-Phosphate Synthase Gene,MdMIPS1,Enhances Heat Stress Tolerance in Transgenic Apple Plants
摘要
Abstract
In order to reveal the role of Myo-inositol(MI)in apple plants responding to high-temperature stress,apple plants were first exogenously supplied with MI and treated with high temperature stress.The results showed that high-temperature stress significantly caused leaf yellow and wilting,increased the contents of reactive oxygen species(ROS),malondialdehyde(MDA),and relative electrolyte leakage(REL),decreased the contents of the chlorophyll in apple plants.However,100 µmol·L-1 exogenous MI treatment significantly alleviated the damage caused by high temperature.In addition,MdMIPS1 encoding the key rate-limiting enzyme in the MI biosynthesis,expressed highly under high-temperature stress in apple plants.The transgenic lines overexpressed MdMIPS1(OE,high MI content)were obtained in our previous study.They had higher endogenous content of MI,compared with wild type(WT)plants.And these OE lines were also used in this work and treated with high temperature stress.Compared with WT plants under high temperature stress,the OE lines exhibited slighter chlorisis and wilting in leaves.The contents of REL were lower and the contents of the chlorophyll were higher in OE plants than in WT.In addition,the stomatal aperture were larger in OE leaves,and the photosynthetic efficiency were stronger in OE plants than in WT.Meanwhile,the OE plants possessed less ROS accumulation and higher antioxidant enzyme activity than WT.The expression levels of several genes encoding the heat shock protein were also higher in OE plants than in WT under high temperature stress.In sum,all these results indicated that increasing the MI levels can significantly enhance tolerance to high temperature stress in apple plants.关键词
苹果/肌醇/MdMIPS1/高温胁迫Key words
apple/Myo-inositol/MdMIPS1/high-temperature stress分类
农业科技引用本文复制引用
孙文静,李小艳,张静允,周辉,黄小军,马锋旺,龚小庆..苹果肌醇-1-磷酸合成酶基因MdMIPS1参与高温胁迫应答的功能分析[J].园艺学报,2025,52(12):3143-3156,14.基金项目
国家自然科学基金项目(32472667,32372648) （32472667,32372648）
现代农业产业技术体系建设专项资助(CARS-27) （CARS-27）
西北农林科技大学教育发展基金会项目(ZJ2023020) （ZJ2023020）
