园艺学报2025，Vol.52Issue(12)：3167-3179,13.DOI:10.16420/j.issn.0513-353x.2024-1034
枳DNA甲基化修饰酶基因的鉴定与表达特征
Genomic-Wide Identification and Expression Analysis of the DNA Methylation-Modifying Enzyme Genes in Poncirus trifoliata
摘要
Abstract
In order to understand the characteristics of DNA methylation-modifying enzyme genes in the citrus genome,the DNA methyltransferase and demethylase genes in Poncirus trifoliata were identified and analyzed at the genome-wide level in this study.The results showed that 11 DNA methyltransferase genes and 3 demethylase genes existed in the Poncirus trifoliata genome.The peptide sequences of those proteins contain 665-1 962 amino acids,and all of them were hydrophilic proteins,with 13 members located at the nucleus.In addition,three fragment duplication events occurred in the PtrDME,PtrROS1,PtrDML3,PtrCMT2,and PtrMET1-6 genes,which were covariant with both Arabidopsis thaliana and Oryza sative.Further analysis revealed that promoter regions of these DNA methylation-modifying genes contain numerous light-responsive and hormone-responsive elements and that the PtrMET1 was a core protein in the protein interaction network.Gene expression analysis demonstrated that PtrMET1-2 and PtrCMT1 were highly expressed in leaf,root,late-stage ovule,and early-stage ovule,while PtrCMT2 and PtrCMT3 were highly expressed in flesh of mature and young fruit and seed.The DNA methylation inhibitor 5-azacitidine treatment reduced the leaf DNA methylation level in Poncirus trifoliata and increased the expression of most DNA methylation-modifying enzyme genes with the exception of PtrRM gene,which showed down expression in the stem.The result indicted that the methylation inhibitor methylation inhibitors may alter the methylation levels of plants via regulating the methylation-related genes expression.关键词
枳/DNA甲基化/生物信息分析/基因表达Key words
Poncirus trifoliata/DNA methylation/bioinformatics analysis/gene expression分类
农业科技引用本文复制引用
陈梦如,吴庆安,何思佳,蔡祥斌,马巧利,辜青青,魏清江..枳DNA甲基化修饰酶基因的鉴定与表达特征[J].园艺学报,2025,52(12):3167-3179,13.基金项目
国家自然科学基金项目(32260751) （32260751）
