园艺学报2025，Vol.52Issue(12)：3189-3201,13.DOI:10.16420/j.issn.0513-353x.2025-0186
青花菜花球性状相关QTL定位及其候选基因分析
QTL Mapping and Candidate Gene Analysis of Related Traits of Broccoli
摘要
Abstract
Using two high-generation inbred broccoli lines(B20101 and B736)exhibiting significant differences in curd agronomic traits as parents,a test population of 228 F2 individuals was obtained through hybridization and selfing.By mapping QTLs related to curd agronomic traits,candidate genes were identified to lay a theoretical foundation for elucidating the molecular mechanisms of curd formation in broccoli and breeding new superior varieties.Based on resequencing technology,SNP markers and constructed a genetic map consisting of 985 bin markers(comprising 6 329 SNP markers)were developed.The map spanned 788.78 cM,with an average marker interval of 0.84 cM.Through QTL mapping analysis of curd-related agronomic traits,3,3,1,4,and 8 QTLs were detected to control stem diameter,curd diameter,curd height,single curd weight,and floret size,respectively.The phenotypic variation explained(PVE)by these QTLs ranged from 4.5689%to 21.0997%.Among them,one major QTL(LOD=10.6897,PVE=21.0997%)controlling floret size was mapped within an approximately 58.977 kb interval defined by SNP markers mk_18639516 and mk_18698493.Analysis of this QTL interval identified four candidate genes-Bo6g067260,Bo6g067270,Bo6g067280,and Bo6g067290-that may influence floret size in the parental lines.These genes showed significant differences in expression levels between the two parents but no sequence variations in the parental lines or the F2 population.This suggests that variations in the regulatory regions(e.g.,promoters)of these genes may lead to differences in gene expression,thereby affecting floret size.关键词
青花菜/农艺性状/遗传图谱/QTL定位/候选基因Key words
broccoli/agronomic traits/genetic map/QTL mapping/candidate genes分类
农业科技引用本文复制引用
高旭,何道根,檀国印,朱长志,章红运,肖家睿,王辉..青花菜花球性状相关QTL定位及其候选基因分析[J].园艺学报,2025,52(12):3189-3201,13.基金项目
浙江省农业(蔬菜)新品种选育重大科技专项(2021C02065) （蔬菜）
台州市重大农业科技"揭榜挂帅"研发攻关计划项目(2022-1) （2022-1）
台州市科技计划项目(22nya02) （22nya02）
台州市农业科技项目(202204,202306,202304,202401) （202204,202306,202304,202401）
