园艺学报2025，Vol.52Issue(12)：3229-3242,14.DOI:10.16420/j.issn.0513-353x.2025-0092
番茄SlPLATZ18调控低温胁迫应答和ABA信号响应的功能分析
Function Analysis of SlPLATZ18 in Regulating Low Temperature Stress and ABA Signal Response in Tomato
摘要
Abstract
PLATZ transcription factors play crucial roles in plant response to abiotic stress.The open reading frame(ORF)of SlPLATZ18 was cloned from'Micro-Tom'tomato.The length of ORF was 687 bp,encoding 228 amino acids.Sequence alignment and phylogenetic tree analysis revealed that SlPLATZ18 had higher homology with StPLATZ9/11 and GhPLATZ1.The SlPLATZ18-overexpressing tomato lines(SlPLATZ18-OE)were generated via genetic transformation.Under low temperature stress,compared to the wild type(WT),SlPLATZ18-OE plants exhibited reduced the accumulation of reactive oxygen species(ROS),enhanced the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),increased the content of proline(Pro),decreased the content of malondialdehyde(MDA)and the relative conductivity.Additionally,the expressions of the cold-responsive genes(SlCBF1,SlCBF3,SlCOR47-like,and SlRD29A)were significantly upregulated in SlPLATZ18-OE than those of WT.The cold tolerance of SlPLATZ18-OE plants was markedly improved.Furthermore,under ABA treatment,the seed germination rates and primary root lengths of SlPLATZ18-OE were significantly higher than those of WT.It was speculated that SlPLATZ18 positively regulated low temperature stress response and negatively regulated ABA signal response in tomato.关键词
番茄/SlPLATZ18/低温/ABAKey words
tomato/SlPLATZ18/low temperature/ABA分类
农业科技引用本文复制引用
樊蓓,任敏,王延峰,陈国梁,党峰峰,程国亭,孙会茹..番茄SlPLATZ18调控低温胁迫应答和ABA信号响应的功能分析[J].园艺学报,2025,52(12):3229-3242,14.基金项目
延安大学博士科研启动项目(YDBK2019-42) （YDBK2019-42）
陕西省自然科学基础研究计划项目(2022JQ-159) （2022JQ-159）
陕西省大学生创新创业训练计划项目(S202310719073) （S202310719073）
