中国农学通报2025，Vol.41Issue(35)：1-8,8.
微生物菌剂与海藻肥联合施用对烟叶品质及叶际微生物的影响
Effects of Combined Application of Microbial Agents and Seaweed Fertilizer on Tobacco Leaf Quality and Phyllosphere Microorganisms
摘要
Abstract
To study the effects of combined application of Rhodopseudomonas palustris,Bacillus velezensis,and seaweed-derived organic water-soluble fertilizer on tobacco leaf quality and phyllosphere microbial ecology,a field plot experiment was conducted with 6 treatments:5×109 cfu/mL Rhodopseudomonas palustris GJ-22 diluted 300×(treatment 1,Rho);5×109 cfu/mL R.palustris GJ-22(300×)+150 g/L seaweed organic water-soluble fertilizer(1000×)(treatment 2,Rhor);5×109 cfu/mLB.velezensis(300×)(treatment 3,Bav);5×109 cfu/mL B.velezensis(300×)+150 g/L seaweed fertilizer(1000×)(treatment 4,Baor);150 g/L seaweed fertilizer(1000×)(treatment 5,ORF);blank control(treatment 6,CK).Agronomic traits and main chemical components of tobacco were measured,and changes in the phyllosphere microbial community structure were analyzed.The results showed that treatment 2 significantly promoted the growth of flue-cured tobacco,increased the relative proportion of C3F and C4F grades,and markedly improved the leaf quality;treatment 1 significantly increased total sugar content in leaves;treatment 2 and 4 significantly increased the relative abundance of Gammaproteobacteria while reducing that of Bacteroidia in the phyllosphere;treatment 1 tended to increase the bacterial and fungal diversity in the phyllosphere,but its combination with organic water-soluble fertilizer did not significantly affect microbial diversity;treatment 3 had no significant effect on the bacterial diversity but significantly reduced fungal diversity;compared with treatment 5,it also significantly reduced the bacterial diversity in the phyllosphere.These findings provide a theoretical basis for the field application of microbial agents and organic water-soluble fertilizers.关键词
沼泽红假单胞菌/贝莱斯芽孢杆菌/海藻有机肥/烟叶品质/叶际微生物Key words
Rhodopseudomonas palustris/Bacillus velezensis/seaweed organic fertilizer/tobacco quality/phyllosphere microorganisms分类
农业科技引用本文复制引用
陈黎,杜晓华,孙书娥,范才银,苏亮,胡亚军,张德咏,朱春晖,封卫平,周劲东,程菊娥..微生物菌剂与海藻肥联合施用对烟叶品质及叶际微生物的影响[J].中国农学通报,2025,41(35):1-8,8.基金项目
湖南省烟草公司衡阳市公司项目"烟稻轮作生产力与土壤健康协同提升技术研究与示范"(HYYC2023KJ28) （HYYC2023KJ28）
湖南省农业科技创新资金项目"水稻微生物组组装关键调控基因Os4CL1的作用机制与抗稻瘟病机制"(2024CX36). （2024CX36）
