中国动物检疫2025，Vol.42Issue(12)：34-41,8.DOI:10.3969/j.issn.1005-944X.2025.12.006
贵阳市1株G9P[13]型猪轮状病毒的分离鉴定与遗传进化分析
Isolation,Identification and Genetic Evolutionary Analysis of One Strain of G9P[13]Type Porcine Rotavirus in Guiyang City of Guizhou Province
摘要
Abstract
In order to confirm the cause of diarrhea affecting a pig herd on a farm in Guiyang City,fecal samples collected from diarrheic pigs were detected for relevant viruses that were subsequently isolated and identified,followed by genetic evolutionary analysis of the virus genes.The results showed that the positivity rate was 67.5％(54/80)for porcine rotavirus(PoRV),but negative results were obtained for porcine epidemic diarrhea virus(PEDV),transmissible gastroenteritis virus(TGEV)and porcine deltacoronavirus(PDCoV).The positive samples were processed and inoculated into MA-104 cells,where stable cytopathic effects appeared after 36 hours.Specific fluorescence was observed by indirect immunofluorescence assay,and one strain of PoRV was successfully isolated.It was found that,by phylogenetic analysis of VP4 and VP7 genes of the isolated strain,the VP4 gene was 96.9％identical to P[13]type murine-derived strain RVA/Murine-tc/CHN/SCLS-M/2018/G3P[13](MK606442);and the VP7 gene was 96.6％homologous to G9 type porcine-derived strain China/GZ/2023(PQ323320),and 88.8％to the U.S.G9 type human-derived strain OM46(AJ491181).Ultimately,the isolated strain was identified as G9P[13]type,and genetically correlative to rotavirus of murine or human origin.A reference was provided for future epidemiological researches and prevention of PoRV infection in China.关键词
猪群腹泻/猪轮状病毒/分离鉴定/遗传进化分析Key words
porcine diarrhea/PoRV/isolation and identification/phylogenetic analysis分类
农业科技引用本文复制引用
张人俊,张晓霞,王晓娇,黄布敏..贵阳市1株G9P[13]型猪轮状病毒的分离鉴定与遗传进化分析[J].中国动物检疫,2025,42(12):34-41,8.基金项目
贵州省科技计划项目(黔科合支撑[2021]一般163,黔科合支撑[2020]1Y037号) （黔科合支撑[2021]一般163,黔科合支撑[2020]1Y037号）
海关总署科研项目(2024HK068) （2024HK068）
