中国全科医学2026，Vol.29Issue(5)：623-630,8.DOI:10.12114/j.issn.1007-9572.2023.0812
自噬相关蛋白Beclin-1及微管相关蛋白1轻链3-Ⅱ在肺癌转移性胸腔积液中的表达研究
Autophagy Related Proteins Beclin-1 and LC3-Ⅱ Expression in Lung Cancer Metaststic Pleural Effusion
摘要
Abstract
Background Malignant pleural effusion,a critical clinical indicator of advanced-stage malignancy and metastatic progression.The existing methods for the diagnosis of the nature of pleural effusion have defects and shortcomings,and it is impossible to timely and effectively determine the nature of pleural effusion.Metastatic lung cancer constitutes the primary etiology of malignant pleural effusion.While the roles of autophagy-related proteins Beclin-1 and LC3-Ⅱ in lung cancer pathogenesis have been well established through multiple studies,their expression patterns and potential as diagnostic biomarkers in MPE urgently require further investigation.Objective To analyze the expression of autophagy-related proteins Beclin-1 and LC3-Ⅱ in lung cancer-associated malignant pleural effusion,and to evaluate their potential value in early clinical diagnosis.Methods From May 2022 to July 2023,we performed bioinformatics analysis of Beclin-1 and LC3 using GEO,GEPIA2,and GeneMANIA databases.Pleural effusion samples were collected from patients who had not received any clinical treatment at the Clinical Laboratory Cell Room of the First Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology,from 2018 to 2023.The collected pleural effusion samples were grouped through liquid-based thin-layer cytology smears combined with clinical data,dividing them into a malignant group(95 cases)and a benign control group(190 cases).The expression of Beclin-1 and LC3-Ⅱ at the gene and protein levels was detected by RT-PCR and Western Blotting methods,respectively,and the expression level of LC3-Ⅱ protein was confirmed using immunofluorescence assay.Results Bioinformatics analysis confirmed the inclusion of Beclin-1 and LC3 in the differentially expressed gene profile of lung cancer.RT-PCR revealed significantly higher mRNA expression levels of both Beclin-1 and LC3-Ⅱ in benign controls versus malignant cases(P<0.05).Western Blotting analysis showed elevated Beclin-1 and LC3-Ⅱ protein abundance in benign specimens compared to malignant group(P<0.05).Immunofluorescence microscopy identified increased FITC-labeled LC3-Ⅱ puncta in benign controls.Conclusion By analyzing the expression differences of autophagy-related proteins Beclin-1 and LC3-Ⅱ in benign pleural effusion and metastatic malignant pleural effusion of lung cancer,may facilitate early diagnosis of MPE and provide novel perspectives for differential diagnosis and targeted therapeutic strategies.关键词
肺肿瘤/胸腔积液/自噬/Beclin-1/LC3-ⅡKey words
Lung neoplasms/Pleural effusion/Autophgy/Beclin-1/LC3-Ⅱ分类
医药卫生引用本文复制引用
姚文静,王翠峰,高金亮,任美英,景学芬,付玉华..自噬相关蛋白Beclin-1及微管相关蛋白1轻链3-Ⅱ在肺癌转移性胸腔积液中的表达研究[J].中国全科医学,2026,29(5):623-630,8.基金项目
内蒙古自然科学基金(2021MS08004) （2021MS08004）
