中国全科医学2026，Vol.29Issue(5)：649-655,7.DOI:10.12114/j.issn.1007-9572.2024.0637
二陈汤干预ATG7/FTH1通路改善痰湿型多囊卵巢综合征大鼠卵泡功能的研究
Study on Erchen Decoction Intervening in ATG7/FTH1 Pathway to Improve Follicular Function of Rats with Phlegm-damp PCOS
摘要
Abstract
Background Polycystic ovary syndrome(PCOS)is a gynecologic endocrine and metabolic disease with no clear cause,the prevalence is getting younger and more prevalent.Therefore,the pathogenesis of PCOS at the molecular biological level was studied to provide relevant evidence for clinical treatment and protect women's health.Objective Based on the theory of"phlegm dampness"constitution leading to infertility in Traditional Chinese Medicine,the ameliorating effect of Erchen decoction on autophagy-dependent ferroptosis in PCOS ovarian granulosa cells was discussed.Methods The Experimental Animal Center of Liaoning University of Traditional Chinese Medicine provided SPF-grade laboratories and 32 female SD rats.After one week of adaptive feeding,32 rats were randomly divided into a control group(n=10)and a model group(n=22).The control group was given regular maintenance feed for the entire duration of 12 weeks.The model group was fed with high-fat feed throughout the whole process,and letrozole solution was gavaged every day during the modeling period.After the modeling was completed,rats with estrous cycle disorders from the model group were selected as the treatment group(n=12),and they were treated with the decoction of Erchen decoction by gavage daily for 4 consecutive weeks.After the samples were collected,the rat ovaries were stained by HE staining to observe the pathological changes,transmission electron microscopy(TEM)was used to show the mitochondrial morphology of ovarian granulosa cells in three groups of rats.Western Blotting was used to detect the changes in ATG7,P62,Beclin1,LC3-Ⅰ,LC3-Ⅱ,LC3-Ⅱ/LC3-Ⅰ,FTH1,ISCU2 protein levels in rat ovarian granulosa cells.Oxygraph-2k mitochondrial function assay system was used to detect changes in mitochondrial oxygen consumption rate in ovarian granulosa cells.Results Ovarian cystic follicle increased in rats of the model group,the corpus luteum was reduced and the mitochondria of ovarian granulosa cells showed autophagy and ferroptosis.Partial recovery of morphology was observed in treatment group after treatment.Compared with the control group,the protein expressions of ATG7,P62,Beclin1 and LC3-Ⅱ in the model group increased(P<0.05),the ratio of LC3-Ⅱ/LC3-Ⅰ increased(P<0.05),and LC3-Ⅰ,FTH1 and ISCU2 protein levels decreased(P<0.05).Compared with the model group,the protein levels of ATG7,P62,Beclin1,and LC3-Ⅱ in the treatment group decreased(P<0.05),the ratio of LC3-Ⅱ/LC3-Ⅰ decreased(P<0.05),and the protein level of FTH1 increased(P<0.05).There was statistical significance in oxygen consumption rates of ETS,CⅠ and CⅡ among the three groups(P<0.05).The oxygen consumption rates of ETS,CⅠ and CⅡ in the model group were lower than those in the control group,and those in the treatment group were higher than those in the model group,with statistical significance(P<0.05).Conclusion Erchen decoction can reverse autophagy-dependent ferroptosis of ovarian granulosa cells through the ATG7/FTH1 pathway,and improve the follicular function of rats with phlegm-damp PCOS.关键词
多囊卵巢综合征/二陈汤/自噬依赖性铁死亡/痰湿不孕/线粒体Key words
Polycystic ovary syndrome/Erchen Decoction/Autophagy-dependent ferroptosis/Phlegm-dampness infertility/Mitochondria分类
医药卫生引用本文复制引用
吕小娇,柏子寒,马妍,陈巍,曲扬,隋国媛,尚冰,丛培玮..二陈汤干预ATG7/FTH1通路改善痰湿型多囊卵巢综合征大鼠卵泡功能的研究[J].中国全科医学,2026,29(5):649-655,7.基金项目
国家自然科学基金青年科学基金项目(82104735) （82104735）
辽宁省科技厅重点研发项目(2024JH2/102500043) （2024JH2/102500043）
辽宁省教育厅青年科技创新团队项目(2024-JYTCB-083) （2024-JYTCB-083）
辽宁省教育厅基础研究面上项目(JYTMS20231815) （JYTMS20231815）
中国博士后科学基金第71批面上资助项目(2022MD713764) （2022MD713764）
中医脏象理论及应用教育部重点实验室开放基金项目(zyzx2202) （zyzx2202）
