中国马铃薯2025，Vol.39Issue(4)：305-311,7.DOI:10.19918/j.cnki.1672-3635.2025.04.008
DAS-ELISA与RT-PCR检测马铃薯病毒结果比较与分析
Comparison and Analysis of DAS-ELISA and RT-PCR Results for Potato Virus Detection
摘要
Abstract
Potato viral diseases are one of the key factors limiting the healthy development of the potato industry.Establishing rapid and accurate detection techniques is an essential prerequisite for controlling these viral diseases.Comparative analysis of double antibody sandwich-enzyme linked immunosorbent assay(DAS-ELISA)and reverse transcription-polymerase chain reaction(RT-PCR)in detecting viral content differences and applicability across potato materials from various sources provides a basis for achieving efficient and rapid detection of viral diseases in samples across different scenarios within the potato industry.DAS-ELISA and RT-PCR were applied to detect various viruses(Potato virus Y,Potato virus S,Potato virus X,Potato virus M,and Potato leafroll virus)in 23 field symptomatic potato samples and 30 micro-apical shoot tip in vitro cultures.Comparative analysis revealed that the results of DAS-ELISA and RT-PCR were highly consistent for symptomatic samples from fields;for latent in vitro cultures,DAS-ELISAshowed poor repeatability in duplicate tests,with concordance rates of 59.33%and 80.66%against RT-PCR,respectively.This study concludes that DAS-ELISA is suitable for high-throughput preliminary screening and identification of field samples with visible virus symptoms,while RT-PCR is appropriate for precise detection of asymptomatic materials such as plantlets in vitro and foundation seed potatoes.关键词
马铃薯/病毒/DAS-ELISA/RT-PCRKey words
potato/virus/DAS-ELISA/RT-PCR分类
农业科技引用本文复制引用
王凤姣,肖乐书,杨丽君,陈光露,胡新喜,秦玉芝..DAS-ELISA与RT-PCR检测马铃薯病毒结果比较与分析[J].中国马铃薯,2025,39(4):305-311,7.基金项目
湖南省现代农业产业技术体系(HARS-02). （HARS-02）
