中国美容医学2026，Vol.35Issue(1)：9-13,5.
米诺地尔酊基于Wnt/β-catenin信号通路治疗雄激素性脱发的药效及机制研究
Study on the Efficacy and Mechanism of Minoxidil Tincture in Treating Androgenetic Alopecia Based on the Wnt/β-catenin Signaling Pathway
摘要
Abstract
Objective To investigate the efficacy and mechanism of minoxidil tincture in treating Androgenetic Alopecia(AGA)based on the Wnt/β-catenin pathway.Methods Mice were randomly assigned into a control group,AGA group,minoxidil tincture group,DKK-1(Wnt/β-catenin pathway inhibitor)group,and minoxidil tincture+DKK-1 group,with 12 mice in each group.Except for the control group,mice in all other groups induced AGA model by applying 0.1 ml of 8%testosterone propionate once a day to the depilation area for 14 consecutive days.On the first day of modeling,each group began to receive medication treatment once a day for 60 days.The hair coverage,hair weight,and hair length in the depilation area of mice were detected.HE staining was applied to observe the status of hair follicles in the depilated skin tissue of mice.QRT-PCR was applied to detect the mRNA expression of Insulin-like Growth Factor(IGF-1)and Vascular Endothelial Growth Factor(VEGF)in the skin tissue of depilated mice.ELISA was applied to detect the levels of dihydrotestosterone,testosterone,Reactive Oxygen Species(ROS),Malondialdehyde(MDA),and Superoxide Dismutase(SOD)activity in the skin tissue of depilated mice.Western blot was applied to detect Wnt3a and β-catenin proteins in the depilated skin tissue of mice.Results Compared with the control group,the majority of hair follicles in the skin tissue of mice in AGA group were located in the epidermis,with fewer follicles,the hair coverage,weight,and length in the depilated area were reduced,the levels of dihydrotestosterone,testosterone,ROS,and MDA in the skin tissue of the hair removal area were increased,the expression of IGF-1 and VEGF mRNA,SOD activity,and Wnt3a and β-catenin proteins in the skin tissue of the depilation area were reduced(P＜0.05).Compared with the AGA group,the mice in the minoxidil tincture group had hair follicles located in the dermis layer,the number of hair follicles increased,and the hair coverage,weight,and length in the hair removal area decreased,the levels of dihydrotestosterone,testosterone,ROS,and MDA in the skin tissue of the hair removal area were reduced,the expression of IGF-1 and VEGF mRNA,SOD activity,and Wnt3a and β-catenin proteins in the skin tissue of the depilation area were elevated,the changes in the above indicators in the DKK-1 group were opposite to those in the minoxidil tincture group(P＜0.05).DKK-1 reversed the effects of minoxidil tincture on androgen levels and oxidative stress in AGA mice.Conclusion Minoxidil tincture may reduce androgen levels,inhibit oxidative stress,and improve mouse AGA by activating the Wnt/β-catenin pathway.关键词
米诺地尔酊/β-连环蛋白/雄激素性脱发/雄激素/氧化应激Key words
minoxidil tincture/β-catenin/androgenetic alopecia/androgen/oxidative stress分类
医药卫生引用本文复制引用
吴美超,姜鹏爽,高晓冉,刘翊,刘冰,石云..米诺地尔酊基于Wnt/β-catenin信号通路治疗雄激素性脱发的药效及机制研究[J].中国美容医学,2026,35(1):9-13,5.基金项目
河北省中医药管理局科研计划项目(编号:2022645) （编号:2022645）
