中国美容医学2026，Vol.35Issue(1)：14-17,62,5.
低能量激光通过激活ROS/ERK通路促进张力诱导下MC3T3-E1成骨分化的机制研究
Mechanism of Low Energy Laser to Promote Stress-induced MC3T3-E1 Osteogenesis Through Activation of ROS/ERK Pathway
摘要
Abstract
Objective This study aimed to investigate the effect of Low-Level Laser Therapy(LLLT)on the function of stress-induced osteogenic differentiation of mouse Embryonic Osteoblasts Cells(MC3T3-E1)and to study the mechanism of action in depth.Methods We divided MC3T3-E1 into control group(CON,no special treatment),stress group(Stress group,subjected to force treatment),laser group(LLLT group,8 J/cm² energy irradiation,irradiated once every other day,each irradiation for 20 s,irradiated for a total of 3 times),and combined group(Stress+LLLT group,laser treatment was performed 2 h after tension treatment).The osteogenic indexes of the cells in each group were detected by alkaline phosphatase(ALP)and Alizarin Red S(ARS)staining.The expression levels of Runt-related transcription factor 2(Runx2),ALP,and osteocalcin(OCN)were detected by qRT-PCR and western blotting.Reactive oxygen species(ROS)and western blotting were utilized to detect changes in the extracellular regulated protein kinases(ERK)pathway.Results The results of ALP and ARS staining showed that both Stress and LLLT treatments alone significantly increased the expression of ALP and ARS,while the combined treatment of the two had a more significant effect.Expression of osteogenesis-related genes and proteins showed that both Stress and LLLT treatment alone increased the expression levels of Runx2 and OCN.In contrast,the combined treatment had a more significant effect.The fluorescence staining and phosphorylation level of ERK showed that both Stress and LLLT treatments alone significantly increased the expression levels of ROS and p-ERK,while the combined treatment had a more significant effect.Conclusion This study concludes that LLLT can activate the ROS/ERK pathway,thereby promoting the osteogenic differentiation of MC3T3-E1 under tension induction.This finding provides an experimental basis for a deeper understanding of the mechanism of LLLT in osteoblastic activity,which has potential application value.关键词
低能量激光治疗/活性氧/张力诱导/成骨分化/信号通路/活性氧分子(ROS)/ERKKey words
low energy laser therapy/reactive oxygen species/stress-induced/osteogenic differentiation/signaling pathway/reactive oxygen species(ROS)/ERK分类
医药卫生引用本文复制引用
李杨,董世涛,周佳星,王坤,王首力..低能量激光通过激活ROS/ERK通路促进张力诱导下MC3T3-E1成骨分化的机制研究[J].中国美容医学,2026,35(1):14-17,62,5.基金项目
承德市科技计划项目(编号:202204A004) （编号:202204A004）
