中国农学通报2025，Vol.41Issue(36)：67-72,6.
观赏石斛兰种间杂交结实性评价
Evaluation of Fruit-Setting Ability in Interspecific Hybridization of Ornamental Dendrobium Orchids
摘要
Abstract
To screen suitable parents for Dendrobium hybrid breeding and clarify the interspecific cross-compatibility as well as parental role bias,20 Dendrobium germplasm resources preserved by the Fuzhou Institute of Agricultural Sciences were used as materials,and 93 interspecific cross combinations were designed.A total of 335 pollination experiments were completed using standardized artificial pollination technology.The number of cross combinations,number of fruit-setting combinations,total number of pollinated flowers,and total number of fruit-setting fruits were counted;the combination fruit-setting rate and pollination fruit-setting rate were calculated.The hybrid fruit-setting ability and parental role bias of each variety were comprehensively evaluated based on the above two indicators.The results showed that:(1)there were significant differences in hybrid fruit-setting ability among different germplasms.Among the 93 combinations,41 cross combinations achieved successful fruit-setting,producing 97 hybrid fruits.Specifically,Dendrobium Oriental Magic'Carmen'and Dendrobium'CT Fairy Kiss'performed optimally when they were used as female parents(combination fruit-setting rate:50.00%-54.55%,pollination fruit-setting rate:53.57%-55.56%),and Dendrobium'Red Star',Dendrobium'Little Blueberry',and Dendrobium'Sunny Red'showed significant advantages when they were used as male parents(combination fruit-setting rate:66.67%-75.00%,pollination fruit-setting rate:52.00%-54.55%).(2)The parental role bias was obvious.Seven varieties exhibited a single parental role bias:Dendrobium'Rainbow Dance Akane',Dendrobium Oriental Magic'Carmen',and Dendrobium'Sweet'were only suitable for female parents(pollination fruit-setting rate≤26.92%when used as male parents);Dendrobium'Red Star',Dendrobium'Splash Ink Pink Fantasy',Dendrobium'Little Blueberry',and Dendrobium'Sunny Red'were only suitable for male parents(pollination fruit-setting rate≤7.14%when used as female parents).Among the 25 reciprocal cross combinations,11 combinations could set fruit bidirectionally,8 combinations could only set fruit unidirectionally,and 6 combinations failed to set fruit in both reciprocal crosses.(3)The core evaluation indicators were clear.The combination fruit-setting rate and pollination fruit-setting rate could synergistically characterize the hybrid fruit-setting ability,and their combination enabled more accurate screening of suitable parents.This study confirms that there are significant differences in interspecific cross-compatibility among ornamental Dendrobium species.The screened excellent parents and the revealed parental role bias provide a scientific basis for parent selection in Dendrobium hybrid breeding,which can effectively improve breeding efficiency.关键词
石斛兰/结实性/杂交/评价/结实率/单向结实/亲本偏向性Key words
Dendrobium/fruit-setting ability/hybridization/evaluation/fruit set rate/one-way fruit set/parental bias分类
农业科技引用本文复制引用
曾凡,魏翠华,周慧君,刘艺,陈芳兰,谢宇,陈沁..观赏石斛兰种间杂交结实性评价[J].中国农学通报,2025,41(36):67-72,6.基金项目
福建省科技计划项目"石斛兰特色种质筛选与创新利用"(2023N0040). （2023N0040）
