中国林副特产Issue(6)：1-4,4.DOI:10.13268/j.cnki.fbsic.2025.06.001
白屈菜红碱及其衍生物对肝癌细胞的抑制作用初探
A Preliminary Study on the Inhibitory Effect of Chelerythrine and Its Derivatives on Hepatocellular Carcinoma Cell
摘要
Abstract
This paper aims to explore the effects of chelerythrine(CHE)and its derivatives dihydrochelerythrine(DHCHE)on the proliferation and apoptosis of hepatocellular carcinoma HepG2 cells.CHE and DHCEH solutions of the same concentration were prepared respectively.The proliferation activity of cells was detected by MTT and colony formation assay,and the changes in cell morphology and intracellular DNA content were observed by micro-scope.The results showed that compared with the negative control group,the inhibition rates of cell growth in both the CHE and DHCHE treatment groups were increased,and the proliferation ability of cells was decreased.Mean-while,the cell morphology of both treatment groups showed signs of rounding and shrinking.The DNA content within the cells was lower than that of the control group,and the CHE group had a greater degree of change than the DHCHE group,exerting a stronger inhibitory effect on the cells.The expression levels of apoptosis-related genes Caspase 3 and TP53 were both upregulated.关键词
白屈菜红碱/二氢白屈菜红碱/增殖/DNAKey words
Chelerythrine/Dihydrochelerythrine/Proliferation/DNA分类
农业科技引用本文复制引用
苏姗,王珏玉,万洪霞,曹柳曦,赵敏,崔岱宗..白屈菜红碱及其衍生物对肝癌细胞的抑制作用初探[J].中国林副特产,2025,(6):1-4,4.基金项目
黑龙江省重点研发计划(创新基地)项目(JD2023SJ14). （创新基地）
