中国兽医科学2025，Vol.55Issue(12)：1574-1583,10.DOI:10.16656/j.issn.1673-4696.2025.0230
DNA双链损伤修复蛋白RAD51在伪狂犬病病毒复制中的功能研究
Function of DNA double-strand break repair protein RAD51 in replication of pseudorabies virus
摘要
Abstract
The aim of this study is to investigate the function of DNA double-strand break repair protein RAD51 in the replication of pseudorabies virus(PRV).Cell lines from various species were infected with PRV,and the RAD51 expression following PRV infection was analyzed by Western-blot,RT-PCR and RT-qPCR.RAD51 overexpression and knockdown A549 cells were generated by lentivirus packaging systems,and the replication of PRV in control and RAD51 overexpression or knockdown cell lines was examined.Meanwhile,the effects of RAD51 specific inhibitors(IBR,B02 and DIDS)on the replication of PRV were evaluated.Western-blot,RT-PCR and RT-qPCR results showed that PRV infection significantly downregulated the expression of RAD51 in various host cells.Western-blot,RT-PCR analysis showed that the PRV replication was significantly enhanced in RAD51 overexpression cell line compared with control cell line,while depletion of RAD51 significantly inhibited the viral replication.In addition,Western-blot,RT-qPCR and RT-PCR data showed that treatment with three RAD51 inhibitors(IBR2,B02 and DIDS)significantly reduced the PRV replication in cells.Collectively,these findings reveal a critical role of RAD51 in facilitating the replication of PRV,and provided new insights into the interaction between PRV infection and host DNA damage response.关键词
伪狂犬病病毒/DNA损伤/RAD51/抑制剂Key words
pseudorabies virus/DNA damage/RAD51/inhibitor分类
农业科技引用本文复制引用
李凤,黄攀锋,杨灵恩,郭桂杰..DNA双链损伤修复蛋白RAD51在伪狂犬病病毒复制中的功能研究[J].中国兽医科学,2025,55(12):1574-1583,10.基金项目
国家自然科学基金面上项目(32372998) （32372998）
