中国兽医科学2025，Vol.55Issue(12)：1584-1590,7.DOI:10.16656/j.issn.1673-4696.2025.0245
鸡传染性法氏囊病病毒及其病毒蛋白对细胞焦亡的影响研究
Studies on the effect of infectious bursal disease virus and its viral proteins on cellular pyroptosis
摘要
Abstract
This study aimed to investigate infectious bursal disease virus(IBDV)-induced cellular pyroptosis and its underlying mechanisms.Cell viability was assessed using the CCK-8 assay,cell death types were analyzed via flow cytometry,and cell membrane integrity was evaluated by lactate dehydrogenase(LDH)release.The results showed that cell viability significantly decreased(P＜0.01),the proportion of Annexin-V/PI double-positive cells increased,and LDH release was elevated at 36 h post-infection.GSDME mRNA expression was significantly upregulated at 24 h post-infection(P＜0.05),indicating the occurrence of pyroptosis.Although transfection with VP2 or VP5 alone did not significantly induce LDH release,both reduced cell viability(P＜0.001).Following IBDV infection,VP2 or VP5 transfection further exacerbated cell damage,suggesting their potential to enhance pyroptosis.Mdivi-1 treatment mitigated pyroptosis,implying that IBDV may regulate this process via the mitochondrial pathway.These findings provide new insight into the pathogenic mechanisms of IBDV.关键词
传染性法氏囊病病毒/细胞焦亡/GSDME/VP2/VP5Key words
infectious bursal disease virus/pyroptosis/GSDME/VP2/VP5分类
农业科技引用本文复制引用
袁柳杨,杨宏春,轩笑然,师雯,陈浩,梁思,欧长波..鸡传染性法氏囊病病毒及其病毒蛋白对细胞焦亡的影响研究[J].中国兽医科学,2025,55(12):1584-1590,7.基金项目
国家自然科学基金项目(32260876) （32260876）
国家现代农业产业体系广西家禽产业创新团队项目(nycytxgxcxtd-2024-19) （nycytxgxcxtd-2024-19）
