中国兽医科学2025，Vol.55Issue(12)：1608-1618,11.DOI:10.16656/j.issn.1673-4696.2025.0215
表达PPV VP2蛋白的重组PRRSV基因工程活载体疫苗株的构建与免疫原性评价
Construction and immunogenicity evaluation of a recombinant porcine reproductive and respiratory syndrome virus genetically engineered live vectored vaccine strain expressing porcine parvovirus VP2 protein
摘要
Abstract
To develop a recombinant live vectored vaccine capable of effectively preventing porcine reproductive and respiratory syndrome(PRRS)and porcine parvovirus(PPV)infection,the reverse genetics platform of the PRRS virus(PRRSV)live vaccine was utilized.The gene encoding the protective antigen protein VP2 of PPV was inserted between the structural and non-structural proteins of PRRSV,constructing a full-length infectious clone of pPRRSV-PPV-VP2.The recombinant virus rPRRSV-PPV-VP2 was rescued in Marc-145 cells,and its plaque morphology,genetic stability,and viral replication kinetics were compared with those of the parental virus.Additionally,piglets were immunized with the recombinant virus to evaluate its ability to elicit immune responses in pigs.The results showed that the VP2 gene of PPV could be stably inherited and efficiently expressed in the recombinant virus rPRRSV-PPV-VP2.The clinical performance of piglets immunized by the recombinant virus rPRRSV-PPV-VP2 was normal,and the autopsy and pathological sections showed that there were no abnormal pathological changes in various tissues and organs,which had good safety for piglets,and at the same time,it could induce piglets to produce specific antibodies against PRRSV and PPV at the same time,which had good immunogenicity.The serum samples of all test pigs on the 0,7th,14th,21st,28th and 35th day after immunization showed that the levels of VP2 protein antibody and N protein antibody turned positive on 7 days after immunization,and the levels of the two antibodies reached their peaks on 21 and 28 days,respectively.The above results indicate that rPRRSV-PPV-VP2 is a prospective live viral vector vaccine against PRRSV and PPV.关键词
猪繁殖与呼吸综合征病毒/猪细小病毒/重组病毒/活载体疫苗Key words
porcine reproductive and respiratory syndrome virus/porcine parvovirus/recombinant virus/live vector vaccine分类
农业科技引用本文复制引用
帅文娜,童武,李兆龙,杨倩,童光志,高飞,唐银保,郭子强,李佳乐,罗梦,曾一凡,李丽薇,周艳君,姜一峰..表达PPV VP2蛋白的重组PRRSV基因工程活载体疫苗株的构建与免疫原性评价[J].中国兽医科学,2025,55(12):1608-1618,11.基金项目
中国农业科学院科技创新工程项目(CAAS-CSLPDCP-202402) （CAAS-CSLPDCP-202402）
上海市兽医生物技术重点实验室开放基金项目(BD1500010) （BD1500010）
国家重点研发计划项目(2022YFD1800804,2021YFD1801401) （2022YFD1800804,2021YFD1801401）
国家自然科学基金项目(32373050,32072861) （32373050,32072861）
