中国药科大学学报2025，Vol.56Issue(6)：758-765,8.DOI:10.11665/j.issn.1000-5048.2025061604
苍术素对LPS诱导的小鼠急性肺损伤的缓解作用及机制
Effect and mechanism of atractyloin LPS-induced acute lung injury in mice
摘要
Abstract
This study aimed to investigate the anti-inflammatory and antioxidant effects of atractylon on lipopolysaccharide(LPS)-induced acute lung injury(ALI)in mice.Changes in lung function parameters were measured in mice after intraperitoneal administration of atractylon.Pathological changes in lung tissue were observed by H&E staining,and the degree of pulmonary edema was assessed by the lung wet/dry weight ratio(W/D).Kit assays were used to detect changes in oxidative stress markers in mouse serum and the protein concentration in bronchoalveolar lavage fluid(BALF).ELISA was employed to measure the expression levels of inflammatory cytokines in BALF and serum.Western blot was used to detect the expression levels of proteins related to the cGAS-STING pathway and vascular cell adhesion molecule-1(VCAM-1)in lung tissue.Results showed that,compared to the ALI model group,mice in the low-dose and high-dose atractylon groups exhibited significant improvement in lung function parameters,alleviated pulmonary edema,and reduced inflammatory cell infiltration in lung tissue.Protein content and inflammatory cytokine levels in serum and BALF were decreased,while serum oxidative stress indicators were improved.Western blot results further indicated that atractylon could regulate the cGAS-STING pathway,blocking the generation of inflammatory signals,and simultaneously inhibit VCAM-1 expression,thereby reducing pulmonary vascular injury.The results suggest that atractylon may alleviate LPS-induced ALI by modulating the cGAS-STING signaling pathway,reducing the expression of pro-inflammatory cytokines and the production of pro-inflammatory mediators,and improving vascular endothelial injury.This study provides a new potential target and theoretical basis for the treatment of ALI,as well as a potential drug candidate for ALI therapy.关键词
苍术素/脂多糖/急性肺损伤/氧化应激/cGAS-STING通路Key words
atractylon/lipopolysaccharide/acute lung injury/oxidative stress/cGAS-STING pathway分类
医药卫生引用本文复制引用
尤梅桂,王鸿苗,汤懿佳,王彩华,许雅苹,钟宏源..苍术素对LPS诱导的小鼠急性肺损伤的缓解作用及机制[J].中国药科大学学报,2025,56(6):758-765,8.基金项目
福建省自然科学基金项目(2024J011399) （2024J011399）
厦门市医疗卫生科技计划项目(3502Z20224ZD1306) （3502Z20224ZD1306）
厦门市医疗卫生指导项目(3502Z20244ZD1155,3502Z20244ZD1259) （3502Z20244ZD1155,3502Z20244ZD1259）
大学生创新创业项目(202412631071) （202412631071）
