中国药房2025，Vol.36Issue(24)：3048-3053,6.DOI:10.6039/j.issn.1001-0408.2025.24.06
芍药汤对湿热泄泻大鼠肠道微生态的影响
Effects of Shaoyao decoction on intestinal microecology in rats with damp-heat diarrhea
摘要
Abstract
OBJECTIVE To explore the impact of Shaoyao decoction on the intestinal microecology of rats with damp-heat diarrhea.METHODS Male SD rats were randomly divided into physiological group,model group,and Shaoyao decoction low-,medium-and high-dose groups(single dose of 5.62,28.10 and 56.20 g/kg,by raw drugs),with 7 rats in each group.Except for the physiological group,the remaining groups were all fed with high-sugar and high-fat diet,raised in a high-temperature and high-humidity environment,and administered white liquor and Escherichia coli suspension by gavage to establish a rat model of damp-heat diarrhea.On the second day after modeling,rats in each dosage group of Shaoyao decoction were administered the corresponding solution by gavage,once in the morning and once in the evening,for 3 consecutive days.After the last administration,fecal metagenomic sequencing analysis and biological information analysis were performed to detect the activities of intestinal cytochrome P450(CYP)enzymes and intestinal flora metabolic enzymes.RESULTS There was no significant difference in the α diversity indexes of intestinal flora among the groups(P＞0.05).The main phyla of rats in each group included Firmicutes,Actinobacteriota and Verrucomicrobiota,and the core dominant genera included Dubosiella,Lactobacillus and Romboutsia;low-,medium-and high-dose of Shaoyao decoction could reverse the decrease in the relative abundance of Firmicutes,Romboutsia,Bifidobacterium and Monoglobus,as well as the increase in the relative abundance of Proteobacteria,Verrucomicrobiota,Escherichia-Shigella and Corynebacterium(P＜0.05).Rats in each dosage group of Shaoyao decoction showed higher relative abundance of intestinal flora involved in carbon metabolism and cofactor biosynthesis pathways.Compared with the model group,the elimination rates of aminophenol and midazolam,as well as the concentration(except for the low-dose group)and metabolic rate of p-nitrophenol in rats of each dose group of Shaoyao decoction were significantly increased(P＜0.05).CONCLUSIONS Shaoyao decoction can correct intestinal flora disorder in rats with damp-heat diarrhea,promote the recovery of intestinal CYP metabolic enzymes and intestinal flora metabolic enzymes,and thereby comprehensively improve intestinal microecology.关键词
芍药汤/湿热泄泻/肠道菌群/细胞色素P450酶/肠道代谢酶/宏基因组学Key words
Shaoyao decoction/damp-heat diarrhea/intestinal flora/CYP enzymes/intestinal flora metabolic enzymes/metagenomics分类
医药卫生引用本文复制引用
凌霄,张丽,张佳楠,李春晓,李学林..芍药汤对湿热泄泻大鼠肠道微生态的影响[J].中国药房,2025,36(24):3048-3053,6.基金项目
国家自然科学基金青年科学基金项目(No.82104726) （No.82104726）
河南省软科学研究项目(No.252400410455) （No.252400410455）
