作物学报2026，Vol.52Issue(1)：279-294,16.DOI:10.3724/SP.J.1006.2026.55044
331份花生种质苗期耐盐性综合评价和强耐盐种质鉴选
Comprehensive evaluation of salt tolerance and identification of elite salt-tolerant germplasm in 331 peanut accessions at seedling stage
摘要
Abstract
Soil salinization is a major constraint limiting the expansion and yield of peanut cultivation.Screening salt-tolerant peanut germplasm and identifying related traits provide a foundation for breeding salt-tolerant varieties and investigating the mechanisms underlying salt tolerance.In this study,the salt tolerance of 331 peanut accessions was evaluated at the seedling stage using a hydroponic system.Eight parameters were measured:SPAD value,plant height,shoot fresh weight,root fresh weight,shoot dry weight,root dry weight,fresh weight root/shoot ratio,and dry weight root/shoot ratio.A comprehensive evaluation of salt tolerance was conducted using principal component analysis(PCA),membership function analysis,and cluster analysis.Un-der different salt concentrations,six parameters—plant height,shoot fresh weight,root fresh weight,shoot dry weight,fresh weight root/shoot ratio,and dry weight root/shoot ratio—showed significant differences among accessions.Salt stress inhibited peanut growth and significantly reduced four of these parameters compared with the control.Correlation analysis revealed significant relationships among the salt tolerance coefficients of all eight parameters,with the strongest correlation observed be-tween root fresh weight and root dry weight(r=0.83).PCA reduced the eight parameters to three principal components,account-ing for a cumulative variance of 76.22%.Based on D-values(comprehensive evaluation scores),cluster analysis grouped the 331 accessions into five categories:Group I(highly salt-tolerant,11 accessions),Group II(salt-tolerant,33 accessions),Group III(intermediate,104 accessions),Group IV(salt-sensitive,42 accessions),and Group V(highly salt-sensitive,141 accessions).A stepwise regression analysis yielded a predictive equation for evaluating salt tolerance in peanut seedlings:Y=0.032+0.163X4+0.137X3+0.073X1-0.158 X2+0.111X5+0.080X6.The D-value proved effective for assessing salt tolerance at the seedling stage,and 11 salt-tolerant germplasms,including Huashi 2,AM-Ceorganic,and Zhonghua 6,were identified.Plant height,root dry weight,and shoot dry weight were identified as key indicators for evaluating peanut salt tolerance at the seedling stage.关键词
花生/苗期/耐盐评价/主成分分析/隶属函数分析/种质鉴选Key words
peanut/seedling stage/assessment of salt tolerance/principal component analysis/subordinate function analysis/germplasm evaluation引用本文复制引用
王菲菲,陈静,张胜忠,杨贵华,苗华荣,胡晓辉,张则林,刘莎莎,乔利仙,单世华..331份花生种质苗期耐盐性综合评价和强耐盐种质鉴选[J].作物学报,2026,52(1):279-294,16.基金项目
本研究由山东省农业良种工程项目(2024LZGC031)和山东省农业科学院创新工程项目(CXGC2025E02)资助. This study was supported by the Shandong Province Agriculture Improved Seed Project(2024LZGC031)and the Innovation Project of Shandong Academy of Agriculture Sciences(CXGC2025E02). （2024LZGC031）
