作物学报2026，Vol.52Issue(1)：316-328,13.DOI:10.3724/SP.J.1006.2026.51043
苦荞半矮秆突变体sd3的表型鉴定及转录组分析
Phenotypic characterization and transcriptomic analysis of the semi-dwarf mutant sd3 in Tartary buckwheat
摘要
Abstract
Plant height is a key agronomic trait in Tartary buckwheat,closely associated with lodging resistance,photosynthetic effi-ciency,and yield formation.Dwarfism is generally advantageous for enhancing lodging resistance and thereby improving yield.To ex-plore dwarf stem gene resources,and to provide a theoretical basis for understanding plant height development and its application in breeding,we conducted phenotypic characterization and transcriptomic analysis of the semi-dwarf mutant sd3,previously generated through EMS mutagenesis.The results showed that compared with the wild type,the plant height of the sd3 at the mature stage reduced 28.43%,and the number of nodes on the main stem decreased by 44.44%.The reduction in node number was the primary cause of the dwarfism in sd3.Cytological observations revealed enhanced lateral cell division at the stem nodes in sd3,which has led to significantly thickened stem walls and an increased basal internode diameter.sd3 is sensitive to gibberellin,and exogenous GA3 can restore its pheno-type.Transcriptome analysis identified 3067 differentially expressed genes(DEGs),GO and KEGG pathway enrichment revealed 57 genes potentially involved in regulating plant height.Notably,GID1 in the gibberellin signaling pathway was upregulated in sd3,while GID2,UNE10,and PIF1 were downregulated,suggesting these genes may play a key role in the dwarf phenotype of sd3.Overall,our findings provide preliminary insights into the dwarfing mechanisms of sd3 and offer valuable candidate genes for breeding lodg-ing-resistant varieties and improving plant architecture through genetic approaches.关键词
苦荞/半矮秆突变体/表型鉴定/转录组/差异表达基因Key words
Tartary buckwheat/semi-dwarf mutant/phenotype identification/transcriptome/differential expression gene引用本文复制引用
刘迪,黎瑞源,石茂竹,李洪有,陈庆富,石桃雄..苦荞半矮秆突变体sd3的表型鉴定及转录组分析[J].作物学报,2026,52(1):316-328,13.基金项目
本研究由国家自然科学基金项目(32260489),财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-07-A5)和贵州省特色杂粮生物育种全省重点实验室项目(黔科合平台ZSYS[2025]026)资助. This study was supported by the National Natural Science Foundation of China(32260489),the China Agriculture Research System of MOF and MARA(CARS-07-A5),and the Guizhou Provincial Key Laboratory of Biology and Breeding for Specialty Crops(QKHPT ZSYS[2025]026). （32260489）
