中药材2025，Vol.48Issue(5)：1200-1205,6.DOI:10.13863/j.issn1001-4454.2025.05.022
基于HPLC指纹图谱和多成分定量评价桑寄生质量
Quality Evaluation of Taxilli Herba Based on HPLC Fingerprint and Multi-component Quantitative Analysis
摘要
Abstract
Objective:To establish the HPLC fingerprint of Taxilli Herba and the determination methods of gallic acid,avicularin,quercitrin,catechin,isoquercitrin and epicatechin,to evaluate the quality of Taxilli Herba from different habitats.Methods:The chroma-tography was performed on the GL Sciences InertSustain AQ-C18(250 mm×4.6 mm,5 μm)chromatographic column,the mobile phase was acetonitrile-0.1%phosphoric acid aqueous solution with gradient elution,detection wavelength was 230 nm,column temperature was 30℃,flow rate was 1.0 mL/min,and injection volume was 10 μL.The HPLC fingerprint of Taxilli Herba was constructed,and the con-tents of six components were simultaneously determined.A combinations of cluster analysis(CA),principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA)and entropy weight-TOPSIS analysis was used to evaluate the quality.Results:HPLC fingerprint of Taxilli Herba was established and 14 common peaks were calibrated.5 markers of quality difference were confirmed.The results of entropy weight-TOPSIS analysis showed that the quality of Taxilli Herba from Guangdong,Guangxi was better.The contents of gallic acid,catechin,epicatechin,isoquercetin,avicularin and quercetin were significantly different in different areas.Conclusion:The HPLC fingerprint and content determination method of Taxilli Herba is reasonable and reliable.Chemometrics combined with entropy weight-TOPSIS method can be used to evaluate the quality of Taxilli Herba and provide basis for its quality control.关键词
桑寄生/指纹图谱/成分含量/质量评价Key words
Taxilli Herba/Fingerprint/Components contents/Quality evaluation分类
医药卫生引用本文复制引用
卓越,王梦蝶,邹丽娜,唐海明,贺海波..基于HPLC指纹图谱和多成分定量评价桑寄生质量[J].中药材,2025,48(5):1200-1205,6.基金项目
湖北省科技厅重点研发大健康计划项目(2022BCE017) （2022BCE017）
湖北省科技厅自然科学基金项目(2022CFB427) （2022CFB427）
湖北省卫生健康委员会中医药重点项目(ZY2023Z015) （ZY2023Z015）
湖北卫生健康委员会卫生健康科研项目(WJ2023M153) （WJ2023M153）
湖北省宜昌市科学技术局医疗卫生研究项目(A23-1-061) （A23-1-061）
