广东海洋大学学报2025，Vol.45Issue(6)：35-43,9.DOI:10.3969/j.issn.1673-9159.2025.06.005
不同剂量四氯化碳和硫代乙酰胺诱导大口黑鲈肝损伤模型的建立
Establishment of Liver Injury Model for Largemouth Bass(Micropterus salmoides)Induced by Different Doses of Carbon Tetrachloride and Thioacetamide
摘要
Abstract
[Objective]This study aimed to establish a stable and reliable liver injury model for largemouth bass(Micropterus salmoides),providing basic data for exploring its liver disease mechanism and developing corresponding prevention and treatment strategies.[Methods]M.salmoides with a body mass of(110±5)g were injected intraperitoneally with CCl4 at volume fractions of 0%,15%,30%,and 45%(denoted as control 1,15%CCl4,30%CCl4,and 45%CCl4 groups,respectively)and thioacetamide(TAA)at doses of 0,0.30,0.45,and 0.60 g/kg(denoted as control 2,0.30 TAA,0.45 TAA,and 0.60 TAA groups,respectively)to induce liver injury.Serum biochemical indices,antioxidant indicators,histopathological changes,and gene expression responses were systematically observed at 1,3,5,and 7 days post-injection.[Results]Both CCl4 and TAA can effectively induce liver injury in M.salmoides,but there are differences in the injury mode and recovery characteristics between the two.At 1,3,5,and 7 days after CCl4 administration,compared to control group 1,the activities of aspartate aminotransferase(AST)and alanine aminotransferase(ALT),and creatinine(CRE)concentrtion significantly increased(P<0.05),while the concentration of total cholesterol(TCHO)significantly decreased(P<0.05);the activities of catalase(CAT)and superoxide dismutase(SOD),and glutathione(GSH)mass fraction were significantly reduced(P<0.05),while the molarity of malondialdehyde(MDA)was significantly increased(P<0.05).At 1,3,and 5 days after TAA administration,compared to control group 2,except for the 0.30 TAA group,the activities of AST and ALT,and CRE concentration significantly increased in all other groups(P<0.05);After 7 days of administration,the activities of AST,ALT,and concentration of CRE in the 0.45 TAA group significantly decreased(P<0.05).At 1 and 3 days after TAA administration,the activities of CAT and SOD,as well as the mass fraction of GSH,significantly decreased compared to control group 2(P<0.05).Subsequently,the activities of CAT and SOD in the 0.45 TAA group significantly increased at 7 days after administration(P<0.05).CCl4 and TAA administration could successfully induce IL-1β,IL-10,TNF-α,as well as apoptosis markers caspase 8,caspase 3,and caspase 9.The 30%CCl4 and 45%CCl4 groups caused irreversible liver damage and persistent necrosis on day 1st after administration.The 0.45 TAA and 0.60 TAA treatment groups showed a recovery trend at 7 days after administration,with histological manifestations of early lymphocyte infiltration,mid-term necrosis with bleeding,and dynamic processes of later tissue repair.[Conclusion]Considering model stability,it is recommended to use a 30%volume fraction of CCl4 to establish an acute irreversible liver injury model,and a dose of 0.45 g/kg TAA to establish an acute self-limiting liver injury model.关键词
大口黑鲈/肝损伤模型/四氯化碳/硫代乙酰胺Key words
Micropterus salmoides/liver injury model/carbon tetrachloride/thioacetamide分类
农业科技引用本文复制引用
朱霞,刘永涛,董靖,杨秋红,周顺,艾晓辉,杨移斌..不同剂量四氯化碳和硫代乙酰胺诱导大口黑鲈肝损伤模型的建立[J].广东海洋大学学报,2025,45(6):35-43,9.基金项目
中国水产科学研究院中央级公益性科研院所基本科研业务费(2023TD47) （2023TD47）
