广东海洋大学学报2025，Vol.45Issue(6)：63-72,10.DOI:10.3969/j.issn.1673-9159.2025.06.008
湛江海域海洋微生物的分离培养及抑菌活性筛选
Isolation and Antibacterial Screening of Marine Microorganisms from Zhanjiang Coastal Area
摘要
Abstract
[Objective]To isolate and screen marine bacteria with antibacterial activity from the marine environment,providing important microbial resources for the subsequent exploration of marine-derived bacteriocins.[Methods]Seawater,marine sediment,and fresh seaweed samples were collected from the coastal area of Zhanjiang.Bacteria were isolated using M10 medium,P3 medium,and BHI medium,respectively,and the antibacterial activity of the isolates against Escherichia coli,Pseudomonas aeruginosa,Staphylococcus aureus,and Listeria monocytogenes were determined by the agar well diffusion method.The 16S rRNA gene of strains with antibacterial activity were amplified by PCR,and their phylogeny with closely related species were analyzed.[Results]A total of 496,421,and 649 bacterial strains were isolated from seawater,marine sediment,and fresh seaweed,respectively,of which 49,48,and 60 strains exhibited antibacterial activity.27 strains with broad-spectrum antibacterial activity were screened,including J-19-2,S-2-2,S-3-2,S-6-6,and S-14-1 from seawater;A05-2,A17-2,A20-6,B15-3,C10-4,and C25-3 from marine sediment;and A4-4,A4-5,A4-6,A4-11,A6-5,A7-2,A20-5,A21-4,A21-7,A25-6,B7-1,B25-1,C7-19,C9-11,C15-2,and D2-5 from fresh seaweed.The 16S rRNA phylogenetic analysis showed that 59.26%(16/27)of the marine bacteria with broad-spectrum antibacterial activity belonged to the genus Bacillus.In addition,there were significant differences in bacterial community composition among seawater,marine sediment,and seaweed samples,but Bacillus was the dominant genus among antibacterial strains,accounting for 16.33%,71.92%,and 63.33%,respectively.[Conclusion]The marine environment of the Zhanjiang coastal area possesses abundant microbial resources with antibacterial activity,among which Bacillus genus show the most prominent antibacterial potential.关键词
海洋微生物/鉴定/抑菌效果/细菌素/湛江海域Key words
marine microorganisms/antibacterial activity/bacteriocin/species identification/Zhanjiang coastal area分类
生物科学引用本文复制引用
马驿,朱湛能,郭富城,彭金菊,曾孜然,彭丹阳,贾明媛,陈秀银,刘钰珍,王欣雨,杨馨怡,郑心仪..湛江海域海洋微生物的分离培养及抑菌活性筛选[J].广东海洋大学学报,2025,45(6):63-72,10.基金项目
广东省自然科学基金项目(2023A1515012181) （2023A1515012181）
广东海洋大学国家级大学生创新创业训练项目(202410566008) （202410566008）
2025年广东省普通高校青年创新人才项目(2025KQNCX027) （2025KQNCX027）
2025-2026年度广东省科协青年科技人才培育计划(SKXRC2025400) （SKXRC2025400）
广东海洋大学滨海农业学院杰出青年项目(BH2025JCQN005) （BH2025JCQN005）
