广东海洋大学学报2025，Vol.45Issue(6)：73-80,8.DOI:10.3969/j.issn.1673-9159.2025.06.009
产褐藻胶裂解酶弧菌的筛选、产酶优化及其铜藻降解应用
Screening and Optimization of Alginate Lyase-Producing Vibrio for Enzyme Production and Its Application in Sargassum horneri Biodegradation
摘要
Abstract
[Objective]This study aims to isolate and screen a marine microorganism with high alginate-degrading efficiency,characterize its degradation products,and optimize the fermentation conditions for maximal enzyme production,thereby providing theoretical support for the high-value development and utilization of Sargassum horneri.[Method]Alginate-degrading strains were isolated from seawater using alginate as the sole carbon source.Strains with high alginate lyase activity were rescreened using the halo formation method and 3,5-dinitrosalicylic acid(DNS)colorimetric assay.Thin-layer chromatography(TLC)was employed to identify alginate oligosaccharides in the strain's fermentation broth.The strain was identified through 16S rDNA sequence analysis.Single-factor experiments were conducted to optimize the enzyme production conditions of the strain,and its degradation effect on S.horneri in the seawater environment was investigated.[Result]A highly efficient alginate-degrading strain,MBP_DH9,was isolated and identified as Vibrio sp..During alginate degradation,this strain predominantly produced disaccharide oligosaccharides.Optimal fermentation conditions were determined to be an incubation temperature of 30℃,an initial pH of 6.5,and an alginate mass concentration of 6 g/L.Under these conditions,MBP_DH9 exhibited an alginate lyase activity of 66.6 U/mL,representing a 44.5%increase compared to the pre-optimization level of 46.1 U/mL.Additionally,MBP_DH9 effectively degraded S.horneri in a seawater environment,achieving a 55.21%mass loss of algal residue and an increase in reducing sugar concentration of 0.204 mg/mL.[Conclusion]A high-efficiency alginate-degrading strain,MBP_DH9,belonging to the genus Vibrio,has been successfully isolated.The strain is capable of degrading alginate to produce alginate disaccharide and effectively degrades S.horneri in seawater.These properties highlight its promising potential for large-scale production of alginate lyase and alginate oligosaccharide-based bioproducts.关键词
褐藻胶裂解酶/褐藻寡糖/弧菌属/产酶条件优化/铜藻降解Key words
alginate lyase/alginate oligosaccharides/Vibrio sp./optimization of enzyme-producing conditions/Sargassum horneri degradation分类
生物科学引用本文复制引用
陈志超,陈逍翔,孙夏好,何佳瑶,陈艳..产褐藻胶裂解酶弧菌的筛选、产酶优化及其铜藻降解应用[J].广东海洋大学学报,2025,45(6):73-80,8.基金项目
浙江省自然科学基金(LTGD24H030004) （LTGD24H030004）
舟山市科技计划项目(2023C41009) （2023C41009）
浙江省大学生科技创新活动计划(2024R411A012) （2024R411A012）
