摘要
Abstract
OBJECTIVE To determine the optimal induction concentration from among different concentrations of hypertonic saline in order to establish a mouse model of thirst and conduct a multi-dimensional evaluation.METHODS Male C57BL/6J mice were randomly divided into the control group(NaCl 0.15 mol·L-1)and hypertonic saline treatment groups(NaCl 1 and 2 mol·L-1).Each group was intraperitoneally administered with the corresponding solution at a dose of 5 μL·g-1.Ten minutes after administration,plasma osmolality was measured via the freezing point osmometry,water intake was measured using the weighing method,and water intake behavior was recorded by a lickometer.Real-time dynamic changes in calcium signals of excitatory neurons in the subfornical organ(SFO)were detected using fiber photometry,and the area under the curve(AUC)was used as the indicator to quantify the excitability of neurons.RESULTS Compared with the control group,plasma osmolality of mice in the treatment groups increased significantly in a dose-dependent manner.In the 2 mol·L-1 treat-ment group,water intake of mice increased significantly,and parameters of water intake behavior(licks per burst,licking rate,number of bursts and burst size)all increased significantly,so did the AUC of the calcium signals of excitatory neurons in the SFO at 400 s after injection,in contrast,these indicators only trended upward in the 1 mol·L-1 treatment group,with no statistical significance.CONCLUSION Treat-ment with NaCl at 2 mol·L-1(5 μL·g-1,ip)can help establish a mouse model of thirst,in which water intake is increased,drinking behavior enhanced,plasma osmolarity elevated,and SFO excitatory neuron activity is enhanced.关键词
口渴/动物模型/摄水/血浆渗透压/穹窿下器/光纤记录技术Key words
thirst/animal model/water intake/plasma osmolality/subfornical organ/fiber recording technology分类
医药卫生
