中医临床研究2025，Vol.17Issue(26)：17-26,10.DOI:10.3969/j.issn.1674-7860.2025.26.002
基于网络药理学探讨金栀清目方对干眼症的作用机制
A discussion on the action mechanism of the Jinzhi Qingmu prescription in the treatment of dry eye disease based on network pharmacology
摘要
Abstract
Objective:To explore the potential action mechanism of the Jinzhi Qingmu prescription(金栀清目方)on dry eye disease(DED)by network pharmacology technology.Methods:The effective ingredients of Chinese medicinal materials used were screened from the TCMSP,and the medicine targets were predicted by the Swiss Target Prediction,Swiss ADME database,and chemsrc.com.The disease targets of DED were obtained by GeneCards and OMIM databases,and the intersections were taken with the medicine targets of the Jinzhi Qingmu prescription to obtain the intersection targets.The Chinese medicinal material component-DED-intersection target network diagram and protein-protein interaction network were drawn by the Cytoscape 3.9.1 software and STRING 12.0 database,respectively.GO and KEGG enrichment analyses were performed to obtain the action mechanism of the Jinzhi Qingmu prescription.Results:The effective active ingredients of the Jinzhi Qingmu prescription for treating DED included mainly quercetin,β-sitosterol,and kaempferol.The key targets included EGFR,EGF,PTGS2,AKT1,MAPK1,etc.The Jinzhi Qingmu prescription might act on biological processes such as gene positive regulation,DNA template transcription positive regulation,and cell nucleus and protein binding,and played a role in the treatment of DED mainly by regulating PI3K-Akt signaling pathway and MAPK signaling pathway.Conclusion:This study initially demonstrated that the Jinzhi Qingmu prescription exerted therapeutic effects on DED through multiple components,multiple targets,and multiple pathways,especially possibly through the PI3K-Akt and MAPK related pathways.关键词
网络药理学/金栀清目方/干眼症Key words
Network pharmacology/The Jinzhi Qingmu prescription/Dry eye disease分类
医药卫生引用本文复制引用
吴沛涓,陈向东..基于网络药理学探讨金栀清目方对干眼症的作用机制[J].中医临床研究,2025,17(26):17-26,10.基金项目
湖南省眼科疾病(中医)临床医学研究中心(2023SK4038) （中医）
湖南省中医药管理局研究项目(B2024048) （B2024048）
湖南省教育厅科学研究项目(22C0184) （22C0184）
湖南中医药大学第一附属医院院内优势病种(干眼症)(201915) （干眼症）
湖南中医药大学第一附属医院中医药传承创新专项(2024XYLH342) （2024XYLH342）
湖南省自然科学基金(2025JJ90006). （2025JJ90006）
