中国药理学与毒理学杂志2025，Vol.39Issue(11)：854-862,9.DOI:10.3867/j.issn.1000-3002.2025.08532
不同来源巨噬细胞与类器官共培养技术的研究进展
Recent advances in macrophage-organoid co-culture
王绩儒 1檀英霞 2楼张蓉 3吴成君4
作者信息
- 1. 大连理工大学医学部,辽宁 大连 116024||军事医学研究院,北京 100850
- 2. 军事医学研究院,北京 100850
- 3. 大连理工大学医学部,辽宁 大连 116024
- 4. 康复大学生命科学与健康学院,山东 青岛 266113
- 折叠
摘要
Abstract
Macrophages are critical effector cells involved in maintaining tissue homeostasis,medi-ating immune responses,and regulating inflammatory reactions.Organoids,serving as three-dimen-sional(3D)in vitro models mimicking tissue structure and function,are widely employed for disease modeling and drug screening.However,organoids typically lack immune system components such as macrophages and therefore cannot fully recapitulate the complex immune microenvironment present in vivo.Co-culturing macrophages with organoids to develop physiologically relevant in vitro models has emerged as a current research focus,enhancing the depth of human disease modeling.This review summarizes recent advances in co-culture studies utilizing macrophages derived from human acute monocytic leukemia THP-1 cells,induced pluripotent stem cells(iPSCs),and other cellular sources,discussing their potential applications in reconstructing immune microenvironments,elucidating disease mechanisms,and facilitating personalized therapeutic strategies.关键词
巨噬细胞/类器官/共培养/诱导多能干细胞/THP-1细胞系/体外模型Key words
macrophage/organoid/co-cultivation/induced pluripotent stem cells/THP-1 cell line/in vitro model分类
医药卫生引用本文复制引用
王绩儒,檀英霞,楼张蓉,吴成君..不同来源巨噬细胞与类器官共培养技术的研究进展[J].中国药理学与毒理学杂志,2025,39(11):854-862,9.
