组织工程与重建外科杂志2025，Vol.21Issue(6)：555-560,570,7.DOI:10.3969/j.issn.1673-0364.2025.06.006
脱钙骨-明胶复合支架结合BMSC-软骨细胞共培养技术体内再生软骨的研究
Study on in vivo cartilage regeneration using decalcified bone matrix-gelatin composite scaffold combined with bmsc-chondrocyte co-culture technology
摘要
Abstract
Objective To explore the feasibility of achieving stable in vivo cartilage regeneration using demineralized bone matrix-gelatin(DBM-GT)composite scaffolds combined with BMSC-chondrocyte co-culture technology.Methods DBM-GT composite scaffolds were prepared via gelatin freeze-drying cross-linking technology,and bone marrow mesenchymal stem cells(BMSCs)were seeded to determine cell loading efficiency.Live/dead staining of scaffold-loaded cells was performed to evaluate the role of chondrocytes in regulating BMSC proliferation.Subsequently,a mixture of BMSCs and chondrocytes at a 1∶4 ratio was seeded onto the composite scaffold as the experimental group,while chondrocytes alone were seeded as the control group.After 5 days of in vitro culture,both groups were implanted subcutaneously into nude mice.Post-harvest histological examination was conducted to assess differences in cartilage regeneration between the experimental and control groups.Results The DBM-GT scaffold exhibited superior porous structure compared to the DBM scaffold.Live/dead staining and DNA quantitative analysis demonstrated that chondrocytes effectively promoted BMSC proliferation.No significant difference in cartilage regeneration was observed between the experimental group(BMSC-chondrocyte co-culture)and the control group(chondrocyte-only)in vivo.Conclusion The DBM-GT gelatin scaffold can be successfully fabricated,and its combination with BMSC-chondrocyte co-culture technology can achieve stable subcutaneous cartilage regeneration in nude mice.关键词
脱钙骨基质/明胶/支架材料/共培养/软骨再生Key words
Decalcified bone matri/Gelatin/Scaffold material/Co-culture/Cartilage regeneration分类
医药卫生引用本文复制引用
宋君涛,慈政,郭学强,石硕洋,周广东..脱钙骨-明胶复合支架结合BMSC-软骨细胞共培养技术体内再生软骨的研究[J].组织工程与重建外科杂志,2025,21(6):555-560,570,7.基金项目
国家自然科学基金项目(82472172) （82472172）
新乡医学院组织工程与再生临床医学中心开放课题(重点)项目(2022KFKTZD01). （重点）
